Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2023 Apr 28

PMID 37112468

Authors

Ana-Maria Chiorcea-Paquim

Affiliations

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Abstract

Breast cancer is the second leading cause of cancer deaths in women worldwide; therefore, there is an increased need for the discovery, development, optimization, and quantification of diagnostic biomarkers that can improve the disease diagnosis, prognosis, and therapeutic outcome. Circulating cell-free nucleic acids biomarkers such as microRNAs (miRNAs) and breast cancer susceptibility gene 1 (BRCA1) allow the characterization of the genetic features and screening breast cancer patients. Electrochemical biosensors offer excellent platforms for the detection of breast cancer biomarkers due to their high sensitivity and selectivity, low cost, use of small analyte volumes, and easy miniaturization. In this context, this article provides an exhaustive review concerning the electrochemical methods of characterization and quantification of different miRNAs and BRCA1 breast cancer biomarkers using electrochemical DNA biosensors based on the detection of hybridization events between a DNA or peptide nucleic acid probe and the target nucleic acid sequence. The fabrication approaches, the biosensors architectures, the signal amplification strategies, the detection techniques, and the key performance parameters, such as the linearity range and the limit of detection, were discussed.

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References

Senel M, Dervisevic M, Kokkokoglu F . Electrochemical DNA biosensors for label-free breast cancer gene marker detection. Anal Bioanal Chem. 2019; 411(13):2925-2935. DOI: 10.1007/s00216-019-01739-9. View

Shabaninejad Z, Yousefi F, Movahedpour A, Ghasemi Y, Dokanehiifard S, Rezaei S . Electrochemical-based biosensors for microRNA detection: Nanotechnology comes into view. Anal Biochem. 2019; 581:113349. DOI: 10.1016/j.ab.2019.113349. View

Li L, Liu Z . Detecting prognostic biomarkers of breast cancer by regularized Cox proportional hazards models. J Transl Med. 2021; 19(1):514. PMC: 8686664. DOI: 10.1186/s12967-021-03180-y. View

Tran H, Piro B . Recent trends in application of nanomaterials for the development of electrochemical microRNA biosensors. Mikrochim Acta. 2021; 188(4):128. DOI: 10.1007/s00604-021-04784-3. View

Tran H, Piro B, Reisberg S, Anquetin G, Duc H, Pham M . An innovative strategy for direct electrochemical detection of microRNA biomarkers. Anal Bioanal Chem. 2013; 406(4):1241-4. DOI: 10.1007/s00216-013-7292-4. View

Han S, Liu W, Yang S, Wang R . Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection Based on DNA Origami Nanostructures. ACS Omega. 2019; 4(6):11025-11031. PMC: 6649092. DOI: 10.1021/acsomega.9b01166. View

Coutinho C, Somoza A . MicroRNA sensors based on gold nanoparticles. Anal Bioanal Chem. 2018; 411(9):1807-1824. DOI: 10.1007/s00216-018-1450-7. View

Fong M, Zhou W, Liu L, Alontaga A, Chandra M, Ashby J . Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis. Nat Cell Biol. 2015; 17(2):183-94. PMC: 4380143. DOI: 10.1038/ncb3094. View

Peng Y, Gao Z . Amplified detection of microRNA based on ruthenium oxide nanoparticle-initiated deposition of an insulating film. Anal Chem. 2011; 83(3):820-7. DOI: 10.1021/ac102370s. View

10.

Bai Y, Wu Z, Xu C, Zhang L, Feng J, Pang D . One-to-Many Single Entity Electrochemistry Biosensing for Ultrasensitive Detection of microRNA. Anal Chem. 2019; 92(1):853-858. DOI: 10.1021/acs.analchem.9b03492. View

11.

Wu J, Yang Q, Li Q, Li H, Li F . Two-Dimensional MnO Nanozyme-Mediated Homogeneous Electrochemical Detection of Organophosphate Pesticides without the Interference of HO and Color. Anal Chem. 2021; 93(8):4084-4091. DOI: 10.1021/acs.analchem.0c05257. View

12.

Chiorcea-Paquim A . 8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC-ECD Determination. Molecules. 2022; 27(5). PMC: 8911600. DOI: 10.3390/molecules27051620. View

13.

Chiorcea-Paquim A, Oliveira-Brett A . DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage. Sensors (Basel). 2021; 21(4). PMC: 7915242. DOI: 10.3390/s21041125. View

14.

Liu S, Su W, Li Y, Zhang L, Ding X . Manufacturing of an electrochemical biosensing platform based on hybrid DNA hydrogel: Taking lung cancer-specific miR-21 as an example. Biosens Bioelectron. 2017; 103:1-5. DOI: 10.1016/j.bios.2017.12.021. View

15.

Liu L, Chang Y, Xia N, Peng P, Zhang L, Jiang M . Simple, sensitive and label-free electrochemical detection of microRNAs based on the in situ formation of silver nanoparticles aggregates for signal amplification. Biosens Bioelectron. 2017; 94:235-242. DOI: 10.1016/j.bios.2017.02.041. View

16.

Pohlmann C, Sprinzl M . Electrochemical detection of microRNAs via gap hybridization assay. Anal Chem. 2010; 82(11):4434-40. DOI: 10.1021/ac100186p. View

17.

Wang W, Zhang W, Wu J, Zhou Z, Ma J . miR-522 regulates cell proliferation, migration, invasion capacities and acts as a potential biomarker to predict prognosis in triple-negative breast cancer. Clin Exp Med. 2021; 22(3):385-392. DOI: 10.1007/s10238-021-00757-1. View

18.

Lusi E, Passamano M, Guarascio P, Scarpa A, Schiavo L . Innovative electrochemical approach for an early detection of microRNAs. Anal Chem. 2009; 81(7):2819-22. DOI: 10.1021/ac8026788. View

19.

Chiorcea-Paquim A, Santos P, Eritja R, Oliveira-Brett A . Self-assembled G-quadruplex nanostructures: AFM and voltammetric characterization. Phys Chem Chem Phys. 2013; 15(23):9117-24. DOI: 10.1039/c3cp50866h. View

20.

Liu X, Li X, Gao X, Ge L, Sun X, Li F . A Universal Paper-Based Electrochemical Sensor for Zero-Background Assay of Diverse Biomarkers. ACS Appl Mater Interfaces. 2019; 11(17):15381-15388. DOI: 10.1021/acsami.9b03860. View