Engineering and Implementation of Synthetic Molecular Tools in the Basidiomycete Fungus

Overview

Journal J Fungi (Basel)

Date 2023 Apr 28

PMID 37108934

Authors

Nicole Heucken

Kun Tang

Lisa Husemann

Natascha Hessler

Kira Muntjes

Michael Feldbrugge

Vera Gohre

Matias D Zurbriggen

Affiliations

Soon will be listed here.

Abstract

The basidiomycete is a well-characterized model organism for studying pathogen-host interactions and of great interest for a broad spectrum of biotechnological applications. To facilitate research and enable applications, in this study, three luminescence-based and one enzymatic quantitative reporter were implemented and characterized. Several dual-reporter constructs were generated for ratiometric normalization that can be used as a fast-screening platform for reporter gene expression, applicable to and detection. Furthermore, synthetic bidirectional promoters that enable bicisitronic expression for gene expression studies and engineering strategies were constructed and implemented. These noninvasive, quantitative reporters and expression tools will significantly widen the application range of biotechnology in and enable the in planta detection of fungal infection.

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