Efficiency of Promoters of Human Genes and at Organism Level in a Model

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Apr 28

PMID 37108352

Authors

Polina I Selina

Irina V Alekseenko

Anastasia I Kurtova

Victor V Pleshkan

Elena E Voronezhskaya

Ilya V Demidyuk

Sergey V Kostrov

Affiliations

Soon will be listed here.

Abstract

The identification of tissue-specific promoters for gene therapeutic constructs is one of the aims of complex tumor therapy. The genes encoding the fibroblast activation protein () and the connective tissue growth factor () can function in tumor-associated stromal cells but are practically inactive in normal adult cells. Accordingly, the promoters of these genes can be used to develop vectors targeted to the tumor microenvironment. However, the efficiency of these promoters within genetic constructs remains underexplored, particularly, at the organism level. Here, we used the model of embryos to study the efficiency of transient expression of marker genes under the control of promoters of the , , and immediate early genes of (CMV). Within 96 h after the injection of vectors, the and CMV promoters provided similar equal efficiency of reporter protein accumulation. In the case of the promoter, a high level of reporter protein accumulation was observed only in certain zebrafish individuals that were considered developmentally abnormal. Disturbed embryogenesis was the factor of changes in the exogenous promoter function. The data obtained make a significant contribution to understanding the function of the human and promoters within vectors to assess their potential in gene therapy.

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