Root Extracts Improve Atopic Dermatitis by Regulating Skin Inflammatory and Anti-Oxidative Enzymes in 2,4-Dinitrochlorobenzene (DNCB)-Induced SKH-1 Hairless Mice

Overview

Journal Antioxidants (Basel)

Date 2023 Apr 28

PMID 37107248

Authors

Tae-Young Kim

No-June Park

Hyun Jegal

Jin-Hyub Paik

Sangho Choi

Su-Nam Kim

Min Hye Yang

Affiliations

Soon will be listed here.

Abstract

is widely used pharmacologically in Traditional Chinese Medicine and Ayurvedic medicine as a diuretic, antipyretic, or choleretic and to treat ulcers, snakebites, and edema. Previous studies have shown that phytochemicals from have physiological activities such as anti-inflammatory, anti-tumor, and anti-wrinkle properties. Nevertheless, research on the anti-atopic dermatitis (AD) effect of extract is limited. This study was undertaken to assess the in vitro and in vivo anti-atopic and antioxidant activities of a 95% EtOH extract of roots (NPR). PI-induced RBL-2H3 cells and two typical hapten mice (oxazolone-induced BALB/c mice and 2,4-dinitrochlorobenzene (DNCB)-induced SKH-1 hairless mice) were used to investigate the effect of NPR extract on AD. The expressions of AD-related inflammatory cytokines, skin-related genes, and antioxidant enzymes were analyzed by ELISA, immunoblotting, and immunofluorescence, and skin hydration was measured using Aquaflux AF103 and SKIN-O-MAT instruments. The chemical composition of NPR extract was analyzed using an HPLC-PDA system. In this study, NPR extracts were shown to most efficiently inhibit IL-4 in PI-induced RBL-2H3 cells and AD-like skin symptoms in oxazolone-BALB/c mice compared to its whole and aerial extracts. NPR extract markedly reduced DNCB-induced increases in mast cells, epidermal thickness, IL-4 and IgE expressions, and atopic-like symptoms in SKH-1 hairless mice. In addition, NPR extract suppressed DNCB-induced changes in the expressions of skin-related genes and skin hydration and activated the Nrf2/HO-1 pathway. Three phenolic acids (chlorogenic acid, 3,5-dicaffeoylquinic acid, and 3,4-dicaffeoylquinic acid) were identified by HPLC-PDA in NPR extract. The study shows that NPR extract exhibits anti-atopic activities by inhibiting inflammatory and oxidative stress and improving skin barrier functions, and indicates that NPR extract has potential therapeutic use for the prevention and treatment of AD.

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References

Kaplan M, Mutlu E, Benson M, Fields J, Banan A, Keshavarzian A . Use of herbal preparations in the treatment of oxidant-mediated inflammatory disorders. Complement Ther Med. 2007; 15(3):207-16. DOI: 10.1016/j.ctim.2006.06.005. View

Tomi N, Luger T . The treatment of atopic dermatitis with topical immunomodulators. Clin Dermatol. 2003; 21(3):215-24. DOI: 10.1016/s0738-081x(02)00367-x. View

Montero-Vilchez T, Segura-Fernandez-Nogueras M, Perez-Rodriguez I, Soler-Gongora M, Martinez-Lopez A, Fernandez-Gonzalez A . Skin Barrier Function in Psoriasis and Atopic Dermatitis: Transepidermal Water Loss and Temperature as Useful Tools to Assess Disease Severity. J Clin Med. 2021; 10(2). PMC: 7833436. DOI: 10.3390/jcm10020359. View

Otsuka A, Nomura T, Rerknimitr P, Seidel J, Honda T, Kabashima K . The interplay between genetic and environmental factors in the pathogenesis of atopic dermatitis. Immunol Rev. 2017; 278(1):246-262. DOI: 10.1111/imr.12545. View

Mitsuishi Y, Motohashi H, Yamamoto M . The Keap1-Nrf2 system in cancers: stress response and anabolic metabolism. Front Oncol. 2012; 2:200. PMC: 3530133. DOI: 10.3389/fonc.2012.00200. View

Poljsak B, Suput D, Milisav I . Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev. 2013; 2013:956792. PMC: 3657405. DOI: 10.1155/2013/956792. View

ORegan G, Irvine A . The role of filaggrin in the atopic diathesis. Clin Exp Allergy. 2010; 40(7):965-72. DOI: 10.1111/j.1365-2222.2010.03522.x. View

Heleno S, Martins A, Queiroz M, Ferreira I . Bioactivity of phenolic acids: metabolites versus parent compounds: a review. Food Chem. 2014; 173:501-13. DOI: 10.1016/j.foodchem.2014.10.057. View

Jin H, He R, Oyoshi M, Geha R . Animal models of atopic dermatitis. J Invest Dermatol. 2008; 129(1):31-40. PMC: 2886143. DOI: 10.1038/jid.2008.106. View

10.

Spergel J, Paller A . Atopic dermatitis and the atopic march. J Allergy Clin Immunol. 2003; 112(6 Suppl):S118-27. DOI: 10.1016/j.jaci.2003.09.033. View

11.

Thestrup-Pedersen K . Clinical aspects of atopic dermatitis. Clin Exp Dermatol. 2000; 25(7):535-43. DOI: 10.1046/j.1365-2230.2000.00696.x. View

12.

Elias P, Schmuth M . Abnormal skin barrier in the etiopathogenesis of atopic dermatitis. Curr Allergy Asthma Rep. 2009; 9(4):265-72. DOI: 10.1007/s11882-009-0037-y. View

13.

Furue M, Chiba T, Tsuji G, Ulzii D, Kido-Nakahara M, Nakahara T . Atopic dermatitis: immune deviation, barrier dysfunction, IgE autoreactivity and new therapies. Allergol Int. 2017; 66(3):398-403. DOI: 10.1016/j.alit.2016.12.002. View

14.

Nocchi N, Duarte H, Pereira R, Ungaretti Paleo Konno T, Soares A . Effects of UV-B radiation on secondary metabolite production, antioxidant activity, photosynthesis and herbivory interactions in Nymphoides humboldtiana (Menyanthaceae). J Photochem Photobiol B. 2020; 212:112021. DOI: 10.1016/j.jphotobiol.2020.112021. View

15.

Ji H, Li X . Oxidative Stress in Atopic Dermatitis. Oxid Med Cell Longev. 2016; 2016:2721469. PMC: 4781995. DOI: 10.1155/2016/2721469. View

16.

Armengot-Carbo M, Hernandez-Martin A, Torrelo A . The role of filaggrin in the skin barrier and disease development. Actas Dermosifiliogr. 2014; 106(2):86-95. DOI: 10.1016/j.ad.2013.10.019. View

17.

Leung D, Boguniewicz M, Howell M, Nomura I, Hamid Q . New insights into atopic dermatitis. J Clin Invest. 2004; 113(5):651-7. PMC: 351324. DOI: 10.1172/JCI21060. View

18.

Beyer T, Auf dem Keller U, Braun S, Schafer M, Werner S . Roles and mechanisms of action of the Nrf2 transcription factor in skin morphogenesis, wound repair and skin cancer. Cell Death Differ. 2007; 14(7):1250-4. DOI: 10.1038/sj.cdd.4402133. View

19.

Howell M, Fairchild H, Kim B, Bin L, Boguniewicz M, Redzic J . Th2 cytokines act on S100/A11 to downregulate keratinocyte differentiation. J Invest Dermatol. 2008; 128(9):2248-58. DOI: 10.1038/jid.2008.74. View

20.

Kawakami T, Ando T, Kimura M, Wilson B, Kawakami Y . Mast cells in atopic dermatitis. Curr Opin Immunol. 2009; 21(6):666-78. PMC: 2839879. DOI: 10.1016/j.coi.2009.09.006. View