Comparative Analysis of Epididymis Cauda of Yak Before and After Sexual Maturity

Overview

Journal Animals (Basel)

Date 2023 Apr 28

PMID 37106918

Authors

Ziqiang Ding

Lin Xiong

Xingdong Wang

Shaoke Guo

Mengli Cao

Yandong Kang

Yongfu La

Pengjia Bao

Jie Pei

Xian Guo

Affiliations

Soon will be listed here.

Abstract

Epididymis development is the basis of male reproduction and is a crucial site where sperm maturation occurs. In order to further understand the epididymal development of yak and how to regulate sperm maturation, we conducted a multi-omics analysis. We detected 2274 differential genes, 222 differential proteins and 117 co-expression genes in the cauda epididymis of yak before and after sexual maturity by RNA-seq and proteomics techniques, which included TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2. These high abundance genes are mainly related to cell growth, differentiation, adhesion and sperm maturation, and are mainly enriched via extracellular matrix receptor interaction, protein differentiation and absorption, and lysosome and estrogen signaling pathways. The abnormal expression of these genes may lead to the retardation of epididymal cauda development and abnormal sperm function in yak. In conclusion, through single and combined analysis, we provided a theoretical basis for the development of the yak epididymal cauda, sperm maturation, and screening of key genes involved in the regulation of male yak reproduction.

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