Serological Cross-Reaction Between Six Thiadiazine by Indirect ELISA Test and Their Antimicrobial Activity

Overview

Journal Methods Protoc

Specialty General Medicine

Date 2023 Apr 27

PMID 37104019

Authors

Mishell Ortiz

Hortensia Rodriguez

Elisabetta Lucci

Julieta Coro

Beatriz Pernia

Abigail Montero-Calderon

Francisco Javier Tingo-Jacome

Leslie Espinoza

Lilian M Spencer

Affiliations

Soon will be listed here.

Abstract

Malaria is a parasitic infection caused by a protozoon of the genus , transmitted to humans by female biting mosquitoes of the genus . Chloroquine and its derivates have caused the parasite to develop drug resistance in endemic areas. For this reason, new anti-malarial drugs as treatments are crucial. This work aimed to evaluate the humoral response. with hyper-immune sera, of mice immunized with six derivatives of tetrahydro-(2H)-1,3,5-thiadiazine-2-thione (bis-THTT) by indirect ELISA test. The cross-reactivity between the compounds as antigens and their microbial activity on Gram-positive and Gram-negative bacteria was evaluated. The results of the humoral evaluation by indirect ELISA show that three bis-THTTs react with almost all of the above. Besides, three compounds used as antigens stimulate the BALB/c mice's immune system. The best combination of two antigens as a combined therapy displays similar absorbances between the antigens in the mixture, showing similar recognition by antibodies and their compounds. In addition, our results showed that different bis-THTT presented antimicrobial activity on Gram-positive bacteria, mainly on strains, and no inhibitory activity was observed on the Gram-negative bacteria tested.

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