Twenty Years of Radical SAM! The Genesis of the Superfamily

Overview

Journal ACS Bio Med Chem Au

Publisher American Chemical Society

Specialty Biochemistry

Date 2023 Apr 27

PMID 37101427

Authors

Squire J Booker

Cody T Lloyd

Affiliations

Soon will be listed here.

Citing Articles

Structural Evidence for DUF512 as a Radical -Adenosylmethionine Cobalamin-Binding Domain.

Wang B, Solinski A, Radle M, Peduzzi O, Knox H, Cui J ACS Bio Med Chem Au. 2024; 4(6):319-330.

PMID: 39712206 PMC: 11659888. DOI: 10.1021/acsbiomedchemau.4c00067.

Metabolites from intact phage-infected Synechococcus chemotactically attract heterotrophic marine bacteria.

Henshaw R, Moon J, Stehnach M, Bowen B, Kosina S, Northen T Nat Microbiol. 2024; 9(12):3184-3195.

PMID: 39548345 DOI: 10.1038/s41564-024-01843-2.

S-adenosyl-L-methionine is the unexpected methyl donor for the methylation of mercury by the membrane-associated HgcAB complex.

Zheng K, Rush K, Date S, Johs A, Parks J, Fleischhacker A Proc Natl Acad Sci U S A. 2024; 121(47):e2408086121.

PMID: 39546574 PMC: 11588087. DOI: 10.1073/pnas.2408086121.

Heme homeostasis and its regulation by hemoproteins in bacteria.

Li Y, Han S, Gao H mLife. 2024; 3(3):327-342.

PMID: 39359680 PMC: 11442138. DOI: 10.1002/mlf2.12120.

Methyltransferases from RiPP pathways: shaping the landscape of natural product chemistry.

Schroder M, Pfeiffer I, Mordhorst S Beilstein J Org Chem. 2024; 20:1652-1670.

PMID: 39076295 PMC: 11285071. DOI: 10.3762/bjoc.20.147.

References

Vey J, Drennan C . Structural insights into radical generation by the radical SAM superfamily. Chem Rev. 2011; 111(4):2487-506. PMC: 5930932. DOI: 10.1021/cr9002616. View

Akiva E, Brown S, Almonacid D, Barber 2nd A, Custer A, Hicks M . The Structure-Function Linkage Database. Nucleic Acids Res. 2013; 42(Database issue):D521-30. PMC: 3965090. DOI: 10.1093/nar/gkt1130. View

Knappe J, Schacht J, MOCKEL W, Hopner T, Vetter Jr H, Edenharder R . Pyruvate formate-lyase reaction in Escherichia coli. The enzymatic system converting an inactive form of the lyase into the catalytically active enzyme. Eur J Biochem. 1969; 11(2):316-27. DOI: 10.1111/j.1432-1033.1969.tb00775.x. View

Wagner A, Frey M, Neugebauer F, Schafer W, Knappe J . The free radical in pyruvate formate-lyase is located on glycine-734. Proc Natl Acad Sci U S A. 1992; 89(3):996-1000. PMC: 48372. DOI: 10.1073/pnas.89.3.996. View

Lloyd C, Iwig D, Wang B, Cossu M, Metcalf W, Boal A . Discovery, structure and mechanism of a tetraether lipid synthase. Nature. 2022; 609(7925):197-203. PMC: 9433317. DOI: 10.1038/s41586-022-05120-2. View

Broderick J, Duffus B, Duschene K, Shepard E . Radical S-adenosylmethionine enzymes. Chem Rev. 2014; 114(8):4229-317. PMC: 4002137. DOI: 10.1021/cr4004709. View

Yokoyama K, Li D, Pang H . Resolving the Multidecade-Long Mystery in MoaA Radical SAM Enzyme Reveals New Opportunities to Tackle Human Health Problems. ACS Bio Med Chem Au. 2022; 2(2):94-108. PMC: 9026282. DOI: 10.1021/acsbiomedchemau.1c00046. View

Wang S, Frey P . S-adenosylmethionine as an oxidant: the radical SAM superfamily. Trends Biochem Sci. 2007; 32(3):101-10. DOI: 10.1016/j.tibs.2007.01.002. View

Frey P, Hegeman A, Ruzicka F . The Radical SAM Superfamily. Crit Rev Biochem Mol Biol. 2008; 43(1):63-88. DOI: 10.1080/10409230701829169. View

10.

Bandarian V . Journey on the Radical SAM Road as an Accidental Pilgrim. ACS Bio Med Chem Au. 2022; 2(3):187-195. PMC: 9204691. DOI: 10.1021/acsbiomedchemau.1c00059. View

11.

Sinner E, Marous D, Townsend C . Evolution of Methods for the Study of Cobalamin-Dependent Radical SAM Enzymes. ACS Bio Med Chem Au. 2022; 2(1):4-10. PMC: 8950095. DOI: 10.1021/acsbiomedchemau.1c00032. View

12.

Horitani M, Shisler K, Broderick W, Hutcheson R, Duschene K, Marts A . Radical SAM catalysis via an organometallic intermediate with an Fe-[5'-C]-deoxyadenosyl bond. Science. 2016; 352(6287):822-5. PMC: 4929858. DOI: 10.1126/science.aaf5327. View

13.

Broderick W, Hoffman B, Broderick J . Mechanism of Radical Initiation in the Radical S-Adenosyl-l-methionine Superfamily. Acc Chem Res. 2018; 51(11):2611-2619. PMC: 6324848. DOI: 10.1021/acs.accounts.8b00356. View

14.

Sofia H, Chen G, Hetzler B, MILLER N . Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res. 2001; 29(5):1097-106. PMC: 29726. DOI: 10.1093/nar/29.5.1097. View

15.

Pagnier A, Balci B, Shepard E, Broderick W, Broderick J . [FeFe]-Hydrogenase In Vitro Maturation. Angew Chem Int Ed Engl. 2022; 61(49):e202212074. DOI: 10.1002/anie.202212074. View

16.

Zeng Z, Liu X, Farley K, Wei J, Metcalf W, Summons R . GDGT cyclization proteins identify the dominant archaeal sources of tetraether lipids in the ocean. Proc Natl Acad Sci U S A. 2019; 116(45):22505-22511. PMC: 6842593. DOI: 10.1073/pnas.1909306116. View

17.

Neti S, Sil D, Warui D, Esakova O, Solinski A, Serrano D . Characterization of LipS1 and LipS2 from : Proteins Annotated as Biotin Synthases, which Together Catalyze Formation of the Lipoyl Cofactor. ACS Bio Med Chem Au. 2022; 2(5):509-520. PMC: 9585515. DOI: 10.1021/acsbiomedchemau.2c00018. View

18.

Oberg N, Precord T, Mitchell D, Gerlt J . RadicalSAM.org: A Resource to Interpret Sequence-Function Space and Discover New Radical SAM Enzyme Chemistry. ACS Bio Med Chem Au. 2022; 2(1):22-35. PMC: 9477430. DOI: 10.1021/acsbiomedchemau.1c00048. View

19.

Logan D, Andersson J, SJOBERG B, Nordlund P . A glycyl radical site in the crystal structure of a class III ribonucleotide reductase. Science. 1999; 283(5407):1499-504. DOI: 10.1126/science.283.5407.1499. View

20.

Krebs C, Broderick W, Henshaw T, Broderick J, Huynh B . Coordination of adenosylmethionine to a unique iron site of the [4Fe-4S] of pyruvate formate-lyase activating enzyme: a Mössbauer spectroscopic study. J Am Chem Soc. 2002; 124(6):912-3. DOI: 10.1021/ja017562i. View