Carbonic Anhydrase Inhibitor Modulation of Intraocular Pressure Is Independent of Soluble Adenylyl Cyclase

Overview

Journal J Ocul Pharmacol Ther

Specialties Ophthalmology
Pharmacology

Date 2023 Apr 25

PMID 37097314

Authors

Shakarr V Wiggins

Ryan Schreiner

Jacob Ferreira

Alan D Marmorstein

Lonny R Levin

Jochen Buck

Affiliations

Soon will be listed here.

Abstract

We investigated whether a clinically used carbonic anhydrase inhibitor (CAIs) can modulate intraocular pressure (IOP) through soluble adenylyl cyclase (sAC) signaling. IOP was measured 1 h after topical treatment with brinzolamide, a topically applied and clinically used CAIs, using direct cannulation of the anterior chamber in sAC knockout (KO) mice or C57BL/6J mice in the presence or absence of the sAC inhibitor (TDI-10229). Mice treated with the sAC inhibitor TDI-10229 had elevated IOP. CAIs treatment significantly decreased increased intraocular pressure (IOP) in wild-type, sAC KO mice, as well as TDI-10229-treated mice. Inhibiting carbonic anhydrase reduces IOP independently from sAC in mice. Our studies suggest that the signaling cascade by which brinzolamide regulates IOP does not involve sAC.

Citing Articles

Adenylyl Cyclase in Ocular Health and Disease: A Comprehensive Review.

Thompson P, Vilkelyte V, Woronkowicz M, Tavakoli M, Skopinski P, Roberts H Biology (Basel). 2024; 13(6).

PMID: 38927325 PMC: 11200476. DOI: 10.3390/biology13060445.

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