Hospital-Related Lineage of USA300 Methicillin-Resistant (MRSA) to Cause Bacteremia in Iran

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2023 Apr 25

PMID 37096227

Authors

Masoumeh Navidinia

Samira Zamani

Anis Mohammadi

Shahram Araghi

Chakameh Amini

Behzad Pourhossein

Mehdi Goudarzi

Affiliations

Soon will be listed here.

Abstract

is an important pathogen that causes bloodstream infections. This study is aimed at assessing the genotypic characteristics of strains responsible for bloodstream infections. An epidemiological study was conducted using 85 strains isolated from bloodstream infections. Susceptibility was tested using the broth microdilution method and disk diffusion. All detected methicillin-resistant (MRSA) isolates were confirmed by mecA PCR assays. isolated from bacteremia were characterized using SCCmec, spa, and multilocus sequence typing methods. The prevalence of strains responsible for bloodstream infections was 38.8%. All isolates were MRSA. Multidrug resistance (MDR) was present in 84.7% of isolates. MRSA isolated categorized within six clonal complexes including CC8 (60%), CC22 (22.4%), CC5 (5.9%), CC30 (4.7%), CC45 (4.7%), and CC59 (2.3%). The main lineages found were USA300/CC8-MRSA-IV/t008 (41.2%), followed by ST22-SCCIV/t790 (9.4%), ST239-SCCIII/t037 (7.1%), ST22-SCCIV/t032 (7.1%), ST239-SCCIII/t631 (5.9%), ST239-SCCIII/t860 (5.9%), ST22-SCCIV/t852 (5.9%), ST5-SCCIV/t002 (4.7%), ST45-SCCIV/t038 (4.7%), ST30-SCCIV/t318 (4.7%), ST59-SCCIV/t437 (2.3%), and ST225-SCCII/t045 (1.1%). Resistance to vancomycin amounted to 5.9% of isolates that belonged to ST239-SCCIII/t037 (80%) and ST8-SCCIV/t008 (20%). The emergence of USA300 strains in bloodstream infections in our country is a serious alarm and highlights the significant invasion of this lineage into the healthcare system. MDR patterns among these strains appear to be becoming the biggest problem in healthcare treatment.

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