Profiling of Differentially Expressed Proteins Between Fresh and Frozen-thawed Duroc Boar Semen Using ProteinChip CM10

Overview

Journal J Anim Sci Technol

Date 2023 Apr 24

PMID 37093902

Authors

Yong-Min Kim

Sung-Woo Park

Mi-Jin Lee

Da-Yeon Jeon

Su-Jin Sa

Yong-Dae Jeong

Ha-Seung Seong

Jung-Woo Choi

Shinichi Hochi

Eun-Seok Cho

Hak-Jae Chung

Affiliations

Soon will be listed here.

Abstract

Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.

Citing Articles

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Hwang Y, Lee E, Lim H, Joo S Food Sci Anim Resour. 2023; 43(6):1067-1086.

PMID: 37969318 PMC: 10636221. DOI: 10.5851/kosfa.2023.e63.

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