Effects of Extender Type and Storage Time on Sperm Quality Parameters of Kail Ram Semen Stored at 5 °C

Overview

Journal Trop Anim Health Prod

Publisher Springer

Specialties Tropical Medicine
Veterinary Medicine

Date 2023 Apr 24

PMID 37093355

Authors

Nasir Hameed

Muhammad Zubair

Nasim Ahmad

Aneela Zameer Durrani

Muhammad Irfan-Ur-Rehman Khan

Affiliations

Soon will be listed here.

Abstract

The study was designed to evaluate the effect of different extenders and storage times on sperm quality parameters of extended Kail ram semen. Semen was collected from five adult Kail rams using an artificial vagina. Semen samples with >70% total sperm motility were pooled, diluted with Tris (TR), sodium citrate (SC), and skim milk (SM)-based extenders, and stored at 5 °C. Sperm motility and kinematics, viability, and plasma and acrosomal membrane integrity were assessed every 24 hrs for 120 hrs. Sperm longevity was estimated by placing semen in a water bath at 37°C to assess sperm kinematics. Storage time as the main effect had a negative (P < 0.05) impact on sperm quality parameters. The percentages of total motile (TM), progressive motile (PM), rapid, and medium progressive (MP) motile sperm were similar at 24 hrs of storage for the three extenders. However, semen extended in TR had a higher (P < 0.05) percentage of TM, PM, rapid, and MP sperm compared to SM and SC extenders at 48 hrs of storage. The sperm kinematics (VCL, VSL, VAP, and ALH) of progressive sperm were similar for all three extenders up to 72 hrs of storage. In addition, semen extended in TR had a higher (P < 0.05) percentage of sperm with intact plasma and acrosomal membranes at a storage time of 48 hrs. At 37 °C, the percentage of TM and PM sperm was higher (P < 0.05) in the TR extender than in the SM and SC extenders at 60 minutes and beyond. In conclusion, the Kail ram sperm extended in TR and stored at 5 °C maintained better quality after 48 hrs storage than sperm extended in SM and SC extenders. At 37 °C, sperm extended in TR also retained better quality parameters at 60 min and afterward than sperm extended in SM and SC.

Citing Articles

Effects of different extenders, storage temperatures, and antioxidant supplementation on chilled semen quality: a review.

Hameed N, Akhter S, Souza-Fabjan J, Zubair M, Khan M Trop Anim Health Prod. 2024; 56(2):85.

PMID: 38386220 DOI: 10.1007/s11250-024-03930-2.

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