Genotyping and Molecular Investigation of Plasmid-mediated Carbapenem Resistant Clinical Isolates in Egypt

Overview

Journal AIMS Microbiol

Specialty Microbiology

Date 2023 Apr 24

PMID 37091821

Authors

Kholoud Baraka

Rania Abozahra

Marwa Mohammed Haggag

Sarah M Abdelhamid

Affiliations

Soon will be listed here.

Abstract

Methods: Antimicrobial resistance of sixty carbapenem-resistant strains was evaluated by using the disc diffusion method. Five carbapenemases' genes were amplified by conventional PCR. Genotyping was performed using ERIC-PCR. Gene transformation was performed for the five genes to sensitive isolates. Wild and transformed isolates were genetically investigated using ERIC-PCR and sequencing.

Results: Carbapenem resistance in our isolates was associated with high resistance to all tested antibiotics. The 60 isolates were divided into 6 resistor types. The prevalence of , and genes were 17%, 63%, 93%, 85% and 100%, respectively. Dendrogram analysis showed 57 distinct patterns, arranged in three clusters. The five genes were transformed successfully into sensitive isolates. ERIC profiles of wild and transformed isolates showed cluster A contained all the wild isolates, and cluster B contained all transformed isolates. Genetic sequences of the 5 genes reflected high genetic similarity with the GenBank reference genes before plasmid transformation; however, a distinguishable decrease of genetic similarity was observed after transformation.

Conclusion: Plasmid-mediated carbapenem resistance in and its dissemination among different strains is a real threat to public health.

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