Proteomic Profiling of Protein Expression Changes After 3 Months-exercise in ESRD Patients on Hemodialysis

Overview

Journal BMC Nephrol

Publisher Biomed Central

Specialty Nephrology

Date 2023 Apr 21

PMID 37085769

Authors

Hye Yun Jeong

Hyun-Ju An

Min Ji Sung

Min Heui Ha

Yu Ho Lee

Dong Ho Yang

Tae Young Yang

Dohyun Han

So-Young Lee

Affiliations

Soon will be listed here.

Abstract

The prevalence of chronic kidney disease (CKD) is steadily increasing, and it is a global health burden. Exercise has been suggested to improve physical activity and the quality of life in patients with CKD, eventually reducing mortality. This study investigated the change in physical performance after exercise in dialysis-dependent patients with CKD and analyzed differentially expressed proteins before and after the exercise. Plasma samples were collected at enrollment and after 3 months of exercise. Liquid chromatography with tandem mass spectrometry analysis and data-independent acquisition results were analyzed to determine the significantly regulated proteins. A total of 37 patients on dialysis were recruited, and 16 were randomized to exercise for 3 months. The hand grip strength and the walking speed significantly improved in the exercise group. Proteome analysis revealed 60 significantly expressed proteins after 3 months of exercise. In the protein functional analysis, the significantly expressed proteins were involved in the immune response. Also, some of the key significantly expressed proteins [(M Matrix metallopeptidase 9 (MMP-9), Activin A Receptor Type 1B (ACVR1B), Fetuin B (FETUB)] were validated via an enzyme-linked immunosorbent assay. Our results showed that exercise in dialysis-dependent patients with CKD could improve their physical performance. These results indicated that this beneficial effect of exercise in these populations could be associated with immune response.

References

Hamed G, Fattah M . Clinical Relevance of matrix metalloproteinase 9 in patients with acute coronary syndrome. Clin Appl Thromb Hemost. 2015; 21(8):705-11. DOI: 10.1177/1076029614567309. View

Afsar B, Siriopol D, Aslan G, Eren O, Dagel T, Kilic U . The impact of exercise on physical function, cardiovascular outcomes and quality of life in chronic kidney disease patients: a systematic review. Int Urol Nephrol. 2018; 50(5):885-904. DOI: 10.1007/s11255-018-1790-4. View

Swirski F, Nahrendorf M . Cardioimmunology: the immune system in cardiac homeostasis and disease. Nat Rev Immunol. 2018; 18(12):733-744. DOI: 10.1038/s41577-018-0065-8. View

Morrow J, Cho M, Platig J, Zhou X, DeMeo D, Qiu W . Ensemble genomic analysis in human lung tissue identifies novel genes for chronic obstructive pulmonary disease. Hum Genomics. 2018; 12(1):1. PMC: 5769240. DOI: 10.1186/s40246-018-0132-z. View

Shon S, Park J, Nahrendorf M, Schellingerhout D, Kim J, Kang B . Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis. 2011; 216(1):67-73. DOI: 10.1016/j.atherosclerosis.2011.01.036. View

Aucella F, Valente G, Catizone L . The role of physical activity in the CKD setting. Kidney Blood Press Res. 2014; 39(2-3):97-106. DOI: 10.1159/000355783. View

Greenwood S, Koufaki P, Mercer T, MacLaughlin H, Rush R, Lindup H . Effect of exercise training on estimated GFR, vascular health, and cardiorespiratory fitness in patients with CKD: a pilot randomized controlled trial. Am J Kidney Dis. 2014; 65(3):425-34. DOI: 10.1053/j.ajkd.2014.07.015. View

Tyanova S, Temu T, Sinitcyn P, Carlson A, Hein M, Geiger T . The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods. 2016; 13(9):731-40. DOI: 10.1038/nmeth.3901. View

Toyama K, Sugiyama S, Oka H, Sumida H, Ogawa H . Exercise therapy correlates with improving renal function through modifying lipid metabolism in patients with cardiovascular disease and chronic kidney disease. J Cardiol. 2010; 56(2):142-6. DOI: 10.1016/j.jjcc.2010.06.007. View

10.

Guralnik J, Ferrucci L, Pieper C, Leveille S, Markides K, Ostir G . Lower extremity function and subsequent disability: consistency across studies, predictive models, and value of gait speed alone compared with the short physical performance battery. J Gerontol A Biol Sci Med Sci. 2000; 55(4):M221-31. DOI: 10.1093/gerona/55.4.m221. View

11.

MacKinnon H, Wilkinson T, Clarke A, Gould D, OSullivan T, Xenophontos S . The association of physical function and physical activity with all-cause mortality and adverse clinical outcomes in nondialysis chronic kidney disease: a systematic review. Ther Adv Chronic Dis. 2018; 9(11):209-226. PMC: 6196637. DOI: 10.1177/2040622318785575. View

12.

Morishita S, Tsubaki A, Shirai N . Physical function was related to mortality in patients with chronic kidney disease and dialysis. Hemodial Int. 2017; 21(4):483-489. DOI: 10.1111/hdi.12564. View

13.

Appel M, Zentgraf K, Kruger K, Alack K . Effects of Genetic Variation on Endurance Performance, Muscle Strength, and Injury Susceptibility in Sports: A Systematic Review. Front Physiol. 2021; 12:694411. PMC: 8334364. DOI: 10.3389/fphys.2021.694411. View

14.

Baria F, Kamimura M, Aoike D, Ammirati A, Rocha M, de Mello M . Randomized controlled trial to evaluate the impact of aerobic exercise on visceral fat in overweight chronic kidney disease patients. Nephrol Dial Transplant. 2014; 29(4):857-64. DOI: 10.1093/ndt/gft529. View

15.

Windelinckx A, De Mars G, Huygens W, Peeters M, Vincent B, Wijmenga C . Comprehensive fine mapping of chr12q12-14 and follow-up replication identify activin receptor 1B (ACVR1B) as a muscle strength gene. Eur J Hum Genet. 2010; 19(2):208-15. PMC: 3025799. DOI: 10.1038/ejhg.2010.173. View

16.

Rossi A, Burris D, Lucas F, Crocker G, Wasserman J . Effects of a renal rehabilitation exercise program in patients with CKD: a randomized, controlled trial. Clin J Am Soc Nephrol. 2014; 9(12):2052-8. PMC: 4255415. DOI: 10.2215/CJN.11791113. View

17.

Filipovic T, Gopcevic K, Dimitrijevic S, Hrkovic M, Backovic A, Lazovic M . Effects of 12-Week Exercise Program on Enzyme Activity of Serum Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Female Patients with Postmenopausal Osteoporosis: A Randomized Control Study. Biomed Res Int. 2020; 2020:9758289. PMC: 7011433. DOI: 10.1155/2020/9758289. View

18.

Watson E, Viana J, Wimbury D, Martin N, Greening N, Barratt J . The Effect of Resistance Exercise on Inflammatory and Myogenic Markers in Patients with Chronic Kidney Disease. Front Physiol. 2017; 8:541. PMC: 5532513. DOI: 10.3389/fphys.2017.00541. View

19.

Lv J, Zhang L . Prevalence and Disease Burden of Chronic Kidney Disease. Adv Exp Med Biol. 2019; 1165:3-15. DOI: 10.1007/978-981-13-8871-2_1. View

20.

Campos J, Gomes K, Ferreira J . Impact of exercise training on redox signaling in cardiovascular diseases. Food Chem Toxicol. 2013; 62:107-19. DOI: 10.1016/j.fct.2013.08.035. View