Acute Effect of Low-load Resistance Exercise with Blood Flow Restriction on Oxidative Stress Biomarkers: A Systematic Review and Meta-analysis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 Apr 21

PMID 37083560

Authors

Joao Vitor Ferlito

Nicholas Rolnick

Marcos Vinicius Ferlito

Thiago De Marchi

Rafael Deminice

Mirian Salvador

Affiliations

Soon will be listed here.

Abstract

Background: The purpose of this review was to analyze the acute effects of low-load resistance exercise with blood flow restriction (LLE-BFR) on oxidative stress markers in healthy individuals in comparison with LLE or high-load resistance exercise (HLRE) without BFR.

Materials And Methods: A systematic review was performed in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. These searches were performed in CENTRAL, SPORTDiscus, EMBASE, PubMed, CINAHL and Virtual Health Library- VHL, which includes Lilacs, Medline and SciELO. The risk of bias and quality of evidence were assessed through the PEDro scale and GRADE system, respectively.

Results: Thirteen randomized clinical trials were included in this review (total n = 158 subjects). Results showed lower post-exercise damage to lipids (SMD = -0.95 CI 95%: -1.49 to -0. 40, I2 = 0%, p = 0.0007), proteins (SMD = -1.39 CI 95%: -2.11 to -0.68, I2 = 51%, p = 0.0001) and redox imbalance (SMD = -0.96 CI 95%: -1.65 to -0.28, I2 = 0%, p = 0.006) in favor of LLRE-BFR compared to HLRE. HLRE presents higher post-exercise superoxide dismutase activity but in the other biomarkers and time points, no significant differences between conditions were observed. For LLRE-BFR and LLRE, we found no difference between the comparisons performed at any time point.

Conclusions: Based on the available evidence from randomized trials, providing very low or low certainty of evidence, this review demonstrates that LLRE-BFR promotes less oxidative stress when compared to HLRE but no difference in levels of oxidative damage biomarkers and endogenous antioxidants between LLRE.

Trial Registration: Register number: PROSPERO number: CRD42020183204.

Citing Articles

Oxidative status alteration during aerobic-dominant mixed and anaerobic-dominant mixed effort in judokas.

Gandouzi I, Fekih S, Selmi O, Chalghaf N, Turki M, Ayedi F Heliyon. 2023; 9(10):e20442.

PMID: 37829795 PMC: 10565691. DOI: 10.1016/j.heliyon.2023.e20442.

Blood flow restriction as a potential therapy to restore physical function following COVID-19 infection.

Wedig I, Durocher J, McDANIEL J, Elmer S Front Physiol. 2023; 14:1235172.

PMID: 37546539 PMC: 10400776. DOI: 10.3389/fphys.2023.1235172.

References

Biazon T, Ugrinowitsch C, Soligon S, Oliveira R, Bergamasco J, Borghi-Silva A . The Association Between Muscle Deoxygenation and Muscle Hypertrophy to Blood Flow Restricted Training Performed at High and Low Loads. Front Physiol. 2019; 10:446. PMC: 6479177. DOI: 10.3389/fphys.2019.00446. View

Item F, Nocito A, Thony S, Bachler T, Boutellier U, Wenger R . Combined whole-body vibration, resistance exercise, and sustained vascular occlusion increases PGC-1α and VEGF mRNA abundances. Eur J Appl Physiol. 2012; 113(4):1081-90. DOI: 10.1007/s00421-012-2524-4. View

Zhang Y, Akl E, Schunemann H . Using systematic reviews in guideline development: the GRADE approach. Res Synth Methods. 2018; 10(3. DOI: 10.1002/jrsm.1313. View

Jelicic Kadic A, Vucic K, Dosenovic S, Sapunar D, Puljak L . Extracting data from figures with software was faster, with higher interrater reliability than manual extraction. J Clin Epidemiol. 2016; 74:119-23. DOI: 10.1016/j.jclinepi.2016.01.002. View

Ozaki H, Yasuda T, Ogasawara R, Sakamaki-Sunaga M, Naito H, Abe T . Effects of high-intensity and blood flow-restricted low-intensity resistance training on carotid arterial compliance: role of blood pressure during training sessions. Eur J Appl Physiol. 2012; 113(1):167-74. DOI: 10.1007/s00421-012-2422-9. View

Guzel N, Hazar S, Erbas D . Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. J Sports Sci Med. 2013; 6(4):417-22. PMC: 3794479. View

Balshem H, Helfand M, Schunemann H, Oxman A, Kunz R, Brozek J . GRADE guidelines: 3. Rating the quality of evidence. J Clin Epidemiol. 2011; 64(4):401-6. DOI: 10.1016/j.jclinepi.2010.07.015. View

Nielsen J, Aagaard P, Prokhorova T, Nygaard T, Bech R, Suetta C . Blood flow restricted training leads to myocellular macrophage infiltration and upregulation of heat shock proteins, but no apparent muscle damage. J Physiol. 2017; 595(14):4857-4873. PMC: 5509865. DOI: 10.1113/JP273907. View

Kozakowska M, Pietraszek-Gremplewicz K, Jozkowicz A, Dulak J . The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes. J Muscle Res Cell Motil. 2016; 36(6):377-93. PMC: 4762917. DOI: 10.1007/s10974-015-9438-9. View

10.

Lixandrao M, Ugrinowitsch C, Berton R, Vechin F, Conceicao M, Damas F . Magnitude of Muscle Strength and Mass Adaptations Between High-Load Resistance Training Versus Low-Load Resistance Training Associated with Blood-Flow Restriction: A Systematic Review and Meta-Analysis. Sports Med. 2017; 48(2):361-378. DOI: 10.1007/s40279-017-0795-y. View

11.

Groennebaek T, Jespersen N, Jakobsgaard J, Sieljacks P, Wang J, Rindom E . Skeletal Muscle Mitochondrial Protein Synthesis and Respiration Increase With Low-Load Blood Flow Restricted as Well as High-Load Resistance Training. Front Physiol. 2019; 9:1796. PMC: 6304675. DOI: 10.3389/fphys.2018.01796. View

12.

Cumming K, Ellefsen S, Ronnestad B, Ugelstad I, Raastad T . Acute and long-term effects of blood flow restricted training on heat shock proteins and endogenous antioxidant systems. Scand J Med Sci Sports. 2016; 27(11):1190-1201. DOI: 10.1111/sms.12774. View

13.

Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N . Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol (1985). 2000; 88(1):61-5. DOI: 10.1152/jappl.2000.88.1.61. View

14.

Powers S, Duarte J, Kavazis A, Talbert E . Reactive oxygen species are signalling molecules for skeletal muscle adaptation. Exp Physiol. 2009; 95(1):1-9. PMC: 2906150. DOI: 10.1113/expphysiol.2009.050526. View

15.

Moseley A, Elkins M, van der Wees P, Pinheiro M . Using research to guide practice: The Physiotherapy Evidence Database (PEDro). Braz J Phys Ther. 2019; 24(5):384-391. PMC: 7563998. DOI: 10.1016/j.bjpt.2019.11.002. View

16.

Powers S, Ji L, Kavazis A, Jackson M . Reactive oxygen species: impact on skeletal muscle. Compr Physiol. 2013; 1(2):941-69. PMC: 3893116. DOI: 10.1002/cphy.c100054. View

17.

Neto G, Novaes J, Salerno V, Goncalves M, Batista G, Cirilo-Sousa M . Does a resistance exercise session with continuous or intermittent blood flow restriction promote muscle damage and increase oxidative stress?. J Sports Sci. 2017; 36(1):104-110. DOI: 10.1080/02640414.2017.1283430. View

18.

Li M, Zhao L, Liu J, Liu A, Jia C, Ma D . Multi-mechanisms are involved in reactive oxygen species regulation of mTORC1 signaling. Cell Signal. 2010; 22(10):1469-76. DOI: 10.1016/j.cellsig.2010.05.015. View

19.

Christiansen D, Murphy R, Bangsbo J, Stathis C, Bishop D . Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. Acta Physiol (Oxf). 2018; 223(2):e13045. PMC: 5969286. DOI: 10.1111/apha.13045. View

20.

Petrick H, Pignanelli C, Barbeau P, Churchward-Venne T, Dennis K, van Loon L . Blood flow restricted resistance exercise and reductions in oxygen tension attenuate mitochondrial H O emission rates in human skeletal muscle. J Physiol. 2019; 597(15):3985-3997. DOI: 10.1113/JP277765. View