» Articles » PMID: 37076452

RUVBL1-modulated Chromatin Remodeling Alters the Transcriptional Activity of Oncogenic CTNNB1 in Uveal Melanoma

Overview
Date 2023 Apr 19
PMID 37076452
Authors
Affiliations
Soon will be listed here.
Abstract

Recent years have witnessed an increasing research interest in the therapeutic value of aberrant chromatin regulatory processes in carcinogenesis. Our study was performed to explore the possible carcinogenic mechanism of the chromatin regulator RuvB-like protein 1 (RUVBL1) in uveal melanoma (UVM). The expression pattern of RUVBL1 was retrieved in bioinformatics data. The correlation between RUVBL1 expression and the prognosis of patients with UVM was analyzed in publicly available database. The downstream target genes of RUVBL1 were predicted and further verified by co-immunoprecipitation. The bioinformatics analysis results showed that RUVBL1 may be associated with the transcriptional activity of CTNNB1 by regulating chromatin remodeling, and that RUVBL1 functioned as an independent prognostic factor for patients with UVM. The UVM cells manipulated with RUVBL1 knockdown were introduced for in vitro investigation. CCK-8 assay, flow cytometry, scratch assay, Transwell assay and Western blot analysis were used for detection on the resultant UVM cell proliferation, apoptosis, migration, invasion and cell cycle distribution. In vitro cell experimental data showed that RUVBL1 expression was significantly increased in UVM cells and RUVBL1 knockdown inhibited the proliferation, invasion and migration of UVM cells, accompanied by augmented apoptosis rate and blocked cell cycle progression. To sum up, RUVBL1 enhances the malignant biological characteristics of UVM cells by increasing the chromatin remodeling and subsequent transcription activity of CTNNB1.

Citing Articles

Regulation of the β‑catenin/LEF‑1 pathway by the siRNA knockdown of RUVBL1 expression inhibits breast cancer cell proliferation, migration and invasion.

Zhang X, Cui D, Sun W, Yang G, Wang W, Mi C Oncol Rep. 2024; 53(2).

PMID: 39670302 PMC: 11667213. DOI: 10.3892/or.2024.8855.


The Lyn/RUVBL1 Complex Promotes Colorectal Cancer Liver Metastasis by Regulating Arachidonic Acid Metabolism Through Chromatin Remodeling.

Zhang Z, Gao Y, Qian Y, Wei B, Jiang K, Sun Z Adv Sci (Weinh). 2024; 12(5):e2406562.

PMID: 39665272 PMC: 11792055. DOI: 10.1002/advs.202406562.


RUVBL1 in Clear-Cell Renal Cell Carcinoma: Unraveling Prognostic Significance and Correlation with HIF1A.

Durslewicz J, Wybierala A, Szczepanek S, Antosik P, Jaworski D, Grzanka D Cancers (Basel). 2024; 16(7).

PMID: 38610951 PMC: 11011037. DOI: 10.3390/cancers16071273.

References
1.
Park J, Diefenbach R, Joshua A, Kefford R, Carlino M, Rizos H . Oncogenic signaling in uveal melanoma. Pigment Cell Melanoma Res. 2018; 31(6):661-672. DOI: 10.1111/pcmr.12708. View

2.
Chen J, Liu G, Wu Y, Ma J, Wu H, Xie Z . Correction to: CircMYO10 promotes osteosarcoma progression by regulating miR-370-3p/RUVBL1 axis to enhance the transcriptional activity of β-catenin/LEF1 complex via effects on chromatin remodeling. Mol Cancer. 2020; 19(1):75. PMC: 7155327. DOI: 10.1186/s12943-020-01193-7. View

3.
Zeng Y, Kong Y, Liao L, Zhu H . Involvement of RUVBL1 in WNT/-Catenin Signaling in Oral Squamous Cell Carcinoma. Dis Markers. 2022; 2022:3398492. PMC: 9054432. DOI: 10.1155/2022/3398492. View

4.
Yuan X, Wang Z, Hu Y, Bao F, Yuan P, Zhang C . Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms. Tumour Biol. 2016; 37:16015–16027. DOI: 10.1007/s13277-016-5452-9. View

5.
Bian J, Dannappel M, Wan C, Firestein R . Transcriptional Regulation of Wnt/β-Catenin Pathway in Colorectal Cancer. Cells. 2020; 9(9). PMC: 7564852. DOI: 10.3390/cells9092125. View