Developmental Competence of IVF and SCNT Goat Embryos is Improved by Inhibition of Canonical WNT Signaling

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 Apr 19

PMID 37075045

Authors

Marjan Sadeghi

Mohsen Rahimi Andani

Mehdi Hajian

Nafiseh Sanei

Reza Moradi-Hajidavaloo

Nasrin Mahvash

Farnoosh Jafarpour

Mohammad Hossein Nasr-Esfahani

Affiliations

Soon will be listed here.

Abstract

The specific role of the canonical WNT/β-catenin signaling pathway during the preimplantation development of goat remains unclear. Our objective was to investigate the expression of β-CATENIN, one of the critical components of Wnt signaling pathway, in IVF embryos and compare it with SCNT embryos in goat. In addition, we evaluated the consequence of inhibition of β-catenin using IWR1. Initially, we observed cytoplasmic expression of β-CATENIN in 2 and 8-16 cell stage embryos and membranous expression of β-CATENIN in compact morula and blastocyst stages. Furthermore, while we observed exclusively membranous localization of β-catenin in IVF blastocysts, we observed both membranous and cytoplasmic localization in SCNT blastocysts. We observed that Inhibition of WNT signaling by IWR1 during compact morula to blastocyst transition (from day 4 till day 7 of in vitro culture) increased blastocyst formation rate in both IVF and SCNT embryos. In conclusion, it seems that WNT signaling system has functional role in the preimplantation goat embryos, and inhibition of this pathway during the period of compact morula to blastocyst transition (D4-D7) can improve preimplantation embryonic development.

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