Syk Regulates the Haemocyte Autophagy Through Inducing the MRNA Expressions of Autophagy-related Genes and the Cleavage of LC3 in Oyster Antibacterial Immunity

Overview

Journal Fish Shellfish Immunol Rep

Date 2023 Apr 17

PMID 37065179

Authors

Hongsheng Yao

Jiejie Sun

Tong Zhang

Lingling Wang

Linsheng Song

Affiliations

Soon will be listed here.

Abstract

Spleen tyrosine kinase (Syk) is reported to be involved in activating the autophagy. Recently, a homologue of Syk was identified from Pacific oyster (defined as Syk). In the present study, the molecular characteristics of Syk and its regulation mechanism in autophagy were investigated in oyster . The full-length cDNA of Syk was of 4566 bp with an open reading frame (ORF) of 1989 bp. Syk encoded a polypeptide of 662 amino acids, containing two Src homology 2 (SH2) domains and one tyrosine kinase catalytic (TyrKc) domain. The deduced amino acid sequence of Syk shared low similarity with the previously identified Syks from other species. In the phylogenetic tree, Syk was first clustered with Syk, and then classified into a branch of invertebrate Syks. In Syk-RNAi oysters, the mRNA expressions of LC3, P62, Beclin-1 and ATG5 in haemocytes decreased significantly at 12 h after stimulation. At the same time, the abundance of LC3Ⅱ in haemocytes, and the autophagy rate of haemocytes in Syk-RNAi oysters decreased significantly at 12 h after stimulation All the results collectively suggested that Syk regulated the autophagy through inducing the mRNA expressions of autophagy-related genes and the cleavage of LC3 to defend against bacterial invasion in oysters.

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