Initiating and Imaging Cavitation from Infused Echo Contrast Agents Through the EkoSonic Catheter

Overview

Journal Sci Rep

Specialty Science

Date 2023 Apr 16

PMID 37062767

Authors

Sonya R Kennedy

Maxime Lafond

Kevin J Haworth

Daniel Suarez Escudero

Dan Ionascu

Brion Frierson

Shaoling Huang

Melvin E Klegerman

Tao Peng

David D McPherson

Curtis Genstler

Christy K Holland

Affiliations

Soon will be listed here.

Abstract

Ultrasound-enhanced delivery of therapeutic-loaded echogenic liposomes is under development for vascular applications using the EkoSonic Endovascular System. In this study, fibrin-targeted echogenic liposomes loaded with an anti-inflammatory agent were characterized before and after infusion through an EkoSonic catheter. Cavitation activity was nucleated by Definity or fibrin-targeted, drug-loaded echogenic liposomes infused and insonified with EkoSonic catheters. Passive cavitation imaging was used to quantify and map bubble activity in a flow phantom mimicking porcine arterial flow. Cavitation was sustained during 3-min infusions of Definity or echogenic liposomes along the distal 6 cm treatment zone of the catheter. Though the EkoSonic catheter was not designed specifically for cavitation nucleation, infusion of drug-loaded echogenic liposomes can be employed to trigger and sustain bubble activity for enhanced intravascular drug delivery.

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References

Huang S, Kee P, Kim H, Moody M, Chrzanowski S, MacDonald R . Nitric oxide-loaded echogenic liposomes for nitric oxide delivery and inhibition of intimal hyperplasia. J Am Coll Cardiol. 2009; 54(7):652-9. PMC: 3108325. DOI: 10.1016/j.jacc.2009.04.039. View

Diez-Silva M, Dao M, Han J, Lim C, Suresh S . Shape and Biomechanical Characteristics of Human Red Blood Cells in Health and Disease. MRS Bull. 2010; 35(5):382-388. PMC: 2998922. DOI: 10.1557/mrs2010.571. View

Kwan J, Myers R, Coviello C, Graham S, Shah A, Eleanor Stride . Ultrasound-Propelled Nanocups for Drug Delivery. Small. 2015; 11(39):5305-14. PMC: 4660885. DOI: 10.1002/smll.201501322. View

Bhatnagar S, Schiffter H, Coussios C . Exploitation of acoustic cavitation-induced microstreaming to enhance molecular transport. J Pharm Sci. 2014; 103(6):1903-12. DOI: 10.1002/jps.23971. View

Arvanitis C, Livingstone M, Vykhodtseva N, McDannold N . Controlled ultrasound-induced blood-brain barrier disruption using passive acoustic emissions monitoring. PLoS One. 2012; 7(9):e45783. PMC: 3454363. DOI: 10.1371/journal.pone.0045783. View

Deprez J, Lajoinie G, Engelen Y, De Smedt S, Lentacker I . Opening doors with ultrasound and microbubbles: Beating biological barriers to promote drug delivery. Adv Drug Deliv Rev. 2021; 172:9-36. DOI: 10.1016/j.addr.2021.02.015. View

Haworth K, Raymond J, Radhakrishnan K, Moody M, Huang S, Peng T . Trans-Stent B-Mode Ultrasound and Passive Cavitation Imaging. Ultrasound Med Biol. 2015; 42(2):518-27. PMC: 4698006. DOI: 10.1016/j.ultrasmedbio.2015.08.014. View

Popel A, Johnson P . Microcirculation and Hemorheology. Annu Rev Fluid Mech. 2011; 37:43-69. PMC: 3000688. DOI: 10.1146/annurev.fluid.37.042604.133933. View

Apfel R, Holland C . Gauging the likelihood of cavitation from short-pulse, low-duty cycle diagnostic ultrasound. Ultrasound Med Biol. 1991; 17(2):179-85. DOI: 10.1016/0301-5629(91)90125-g. View

10.

Beekers I, Vegter M, Lattwein K, Mastik F, Beurskens R, van der Steen A . Opening of endothelial cell-cell contacts due to sonoporation. J Control Release. 2020; 322:426-438. DOI: 10.1016/j.jconrel.2020.03.038. View

11.

Aday A, Matsushita K . Epidemiology of Peripheral Artery Disease and Polyvascular Disease. Circ Res. 2021; 128(12):1818-1832. PMC: 8202714. DOI: 10.1161/CIRCRESAHA.121.318535. View

12.

Haworth K, Bader K, Rich K, Holland C, Mast T . Quantitative Frequency-Domain Passive Cavitation Imaging. IEEE Trans Ultrason Ferroelectr Freq Control. 2016; 64(1):177-191. PMC: 5344809. DOI: 10.1109/TUFFC.2016.2620492. View

13.

Badimon L, Vilahur G . Thrombosis formation on atherosclerotic lesions and plaque rupture. J Intern Med. 2014; 276(6):618-32. DOI: 10.1111/joim.12296. View

14.

Szijjarto C, Rossi S, Waton G, Krafft M . Effects of perfluorocarbon gases on the size and stability characteristics of phospholipid-coated microbubbles: osmotic effect versus interfacial film stabilization. Langmuir. 2011; 28(2):1182-9. DOI: 10.1021/la2043944. View

15.

Kabalnov A, Bradley J, Flaim S, Klein D, Pelura T, Peters B . Dissolution of multicomponent microbubbles in the bloodstream: 2. Experiment. Ultrasound Med Biol. 1998; 24(5):751-60. DOI: 10.1016/s0301-5629(98)00033-7. View

16.

Escudero D, Lafond M, Salido N, Haworth K, Hannah A, Macke G . Corrigendum to 'Cavitation emissions nucleated by Definity infused through an EkoSonic catheter in a flow phantom' [Ultrasound in Med & Biol. 47 (2021) 693-709]. Ultrasound Med Biol. 2022; 49(1):410-414. PMC: 11567652. DOI: 10.1016/j.ultrasmedbio.2022.09.012. View

17.

Belcik J, Davidson B, Xie A, Wu M, Yadava M, Qi Y . Augmentation of Muscle Blood Flow by Ultrasound Cavitation Is Mediated by ATP and Purinergic Signaling. Circulation. 2017; 135(13):1240-1252. PMC: 5373943. DOI: 10.1161/CIRCULATIONAHA.116.024826. View

18.

Lattwein K, Shekhar H, van Wamel W, Gonzalez T, Herr A, Holland C . An in vitro proof-of-principle study of sonobactericide. Sci Rep. 2018; 8(1):3411. PMC: 5821825. DOI: 10.1038/s41598-018-21648-8. View

19.

Smith E . Fibrinogen, fibrin and fibrin degradation products in relation to atherosclerosis. Clin Haematol. 1986; 15(2):355-70. View

20.

Smith C, Coussios C . Spatiotemporal Assessment of the Cellular Safety of Cavitation-Based Therapies by Passive Acoustic Mapping. Ultrasound Med Biol. 2020; 46(5):1235-1243. DOI: 10.1016/j.ultrasmedbio.2020.01.009. View