Mosquito Bites and Stage-specific Antibody Responses Against Plasmodium Falciparum in Southern Ghana

Overview

Journal Malar J

Publisher Biomed Central

Specialty Tropical Medicine

Date 2023 Apr 15

PMID 37061695

Authors

Sebastian Shine Kwapong

Kwame Kumi Asare

Kwadwo Asamoah Kusi

Faustina Pappoe

Nicaise Ndam

Rachida Tahar

Anne Poinsignon

Linda Eva Amoah

Affiliations

Soon will be listed here.

Abstract

Background: The human host elicits specific immune responses after exposure to various life stages of the malaria parasite as well as components of mosquito saliva injected into the host during a mosquito bite. This study describes differences in IgG responses against antigens derived from the sporozoite (PfCSP), asexual stage parasite (PfEBA175) and the gametocyte (Pfs230), in addition to an Anopheles gambiae salivary gland antigen (gSG6-P1), in two communities in Ghana with similar blood stage malaria parasite prevalence.

Methods: This study used archived plasma samples collected from an earlier cross-sectional study that enrolled volunteers aged from 6 months to 70 years from Simiw, peri-urban community (N = 347) and Obom, rural community (N = 291). An archived thick and thin blood smear for microscopy was used for the estimation of Plasmodium parasite density and species and DNA extraction from blood spots and P. falciparum confirmation was performed using PCR. This study used the stored plasma samples to determine IgG antibody levels to P. falciparum and Anopheles salivary antigens using indirect ELISA.

Results: Individuals from Simiw had significantly higher levels of IgG against mosquito gSG6-P1 [median (95%CI)] [2.590 (2.452-2.783) ng/mL] compared to those from Obom [2.119 (1.957-2.345) ng/mL], p < 0.0001. Both IgG responses against Pfs230proC (p = 0.0006), and PfCSP (p = 0.002) were significantly lower in volunteers from Simiw compared to the participants from Obom. The seroprevalence of PfEBA-175.5R (p = 0.8613), gSG6-P1 (p = 0.0704), PfCSP (p = 0.7798) IgG were all similar in Obom and Simiw. However, Pfs230 seroprevalence was significantly higher at Obom compared to Simiw (p = 0.0006). Spearman correlation analysis showed no significant association between IgG responses against gSG6-P1, PfCSP, Pfs230proC and PfEBA-175.5R and parasite density at both Obom and Simiw (p > 0.05).

Conclusion: In conclusion, the study showed that participants from Simiw had higher concentrations of circulating gSG6-P1 IgG antibodies but lower concentrations of P. falciparum antibodies, PfCSP IgG and Pfs230proC IgG compared to participants from Obom.

Citing Articles

Plasmodium Falciparum and mosquito vector IgG patterns across suspected malaria cases in Ghana.

Asare K, Kwapong S, Tey P, Sackey V, Nuvor S, Amoah L BMC Infect Dis. 2024; 24(1):1374.

PMID: 39623362 PMC: 11613542. DOI: 10.1186/s12879-024-10248-9.

Immune responses to P falciparum antibodies in symptomatic malaria patients with variant hemoglobin genotypes in Ghana.

Asare K, Agrah B, Ofori-Acquah F, Kudzi W, Aryee N, Amoah L BMC Immunol. 2024; 25(1):14.

PMID: 38336647 PMC: 10858493. DOI: 10.1186/s12865-024-00607-1.

References

Ya-Umphan P, Cerqueira D, Cottrell G, Parker D, Fowkes F, Nosten F . Salivary Biomarker as a Proxy for Estimating Malaria Exposure on the Thailand-Myanmar Border. Am J Trop Med Hyg. 2018; 99(2):350-356. PMC: 6090370. DOI: 10.4269/ajtmh.18-0081. View

Siri J, Wilson M, Murray S, Rosen D, Vulule J, Slutsker L . Significance of travel to rural areas as a risk factor for malarial anemia in an urban setting. Am J Trop Med Hyg. 2010; 82(3):391-7. PMC: 2829898. DOI: 10.4269/ajtmh.2010.09-0047. View

Duffy P . Transmission-Blocking Vaccines: Harnessing Herd Immunity for Malaria Elimination. Expert Rev Vaccines. 2021; 20(2):185-198. PMC: 11127254. DOI: 10.1080/14760584.2021.1878028. View

Jaskiewicz E, Jodlowska M, Kaczmarek R, Zerka A . Erythrocyte glycophorins as receptors for Plasmodium merozoites. Parasit Vectors. 2019; 12(1):317. PMC: 6591965. DOI: 10.1186/s13071-019-3575-8. View

Shapiro L, Murdock C, Jacobs G, Thomas R, Thomas M . Larval food quantity affects the capacity of adult mosquitoes to transmit human malaria. Proc Biol Sci. 2016; 283(1834). PMC: 4947883. DOI: 10.1098/rspb.2016.0298. View

Quelhas D, Puyol L, Quinto L, Serra-Casas E, Nhampossa T, Macete E . Impact of intermittent preventive treatment with sulfadoxine-pyrimethamine on antibody responses to erythrocytic-stage Plasmodium falciparum antigens in infants in Mozambique. Clin Vaccine Immunol. 2008; 15(8):1282-91. PMC: 2519318. DOI: 10.1128/CVI.00044-08. View

Jaramillo-Underwood A, Impoinvil D, Sutcliff A, Hamre K, Joseph V, van den Hoogen L . Factors Associated With Human IgG Antibody Response to Anopheles albimanus Salivary Gland Extract, Artibonite Department, Haiti, 2017. J Infect Dis. 2022; 226(8):1461-1469. PMC: 10982684. DOI: 10.1093/infdis/jiac245. View

Reddy M, Overgaard H, Abaga S, Reddy V, Caccone A, Kiszewski A . Outdoor host seeking behaviour of Anopheles gambiae mosquitoes following initiation of malaria vector control on Bioko Island, Equatorial Guinea. Malar J. 2011; 10:184. PMC: 3146901. DOI: 10.1186/1475-2875-10-184. View

Belachew E . Immune Response and Evasion Mechanisms of Parasites. J Immunol Res. 2018; 2018:6529681. PMC: 5889876. DOI: 10.1155/2018/6529681. View

10.

Abagna H, Acquah F, Okonu R, Aryee N, Theisen M, Amoah L . Assessment of the quality and quantity of naturally induced antibody responses to EBA175RIII-V in Ghanaian children living in two communities with varying malaria transmission patterns. Malar J. 2018; 17(1):14. PMC: 5759240. DOI: 10.1186/s12936-017-2167-3. View

11.

Duffy P, Gorres J . Malaria vaccines since 2000: progress, priorities, products. NPJ Vaccines. 2020; 5(1):48. PMC: 7283239. DOI: 10.1038/s41541-020-0196-3. View

12.

Yan J, Gangoso L, Ruiz S, Soriguer R, Figuerola J, Martinez-de la Puente J . Understanding host utilization by mosquitoes: determinants, challenges and future directions. Biol Rev Camb Philos Soc. 2021; 96(4):1367-1385. DOI: 10.1111/brv.12706. View

13.

Keleta Y, Ramelow J, Cui L, Li J . Molecular interactions between parasite and mosquito during midgut invasion as targets to block malaria transmission. NPJ Vaccines. 2021; 6(1):140. PMC: 8630063. DOI: 10.1038/s41541-021-00401-9. View

14.

Beeson J, Drew D, Boyle M, Feng G, Fowkes F, Richards J . Merozoite surface proteins in red blood cell invasion, immunity and vaccines against malaria. FEMS Microbiol Rev. 2016; 40(3):343-72. PMC: 4852283. DOI: 10.1093/femsre/fuw001. View

15.

Nahum A, Erhart A, Maye A, Ahounou D, Van Overmeir C, Menten J . Malaria incidence and prevalence among children living in a peri-urban area on the coast of benin, west Africa: a longitudinal study. Am J Trop Med Hyg. 2010; 83(3):465-73. PMC: 2929036. DOI: 10.4269/ajtmh.2010.09-0611. View

16.

Mmbando A, Ngowo H, Kilalangongono M, Abbas S, Matowo N, Moore S . Small-scale field evaluation of push-pull system against early- and outdoor-biting malaria mosquitoes in an area of high pyrethroid resistance in Tanzania. Wellcome Open Res. 2018; 2:112. PMC: 5840620. DOI: 10.12688/wellcomeopenres.13006.1. View

17.

Acquah F, Donu D, Obboh E, Bredu D, Mawuli B, Amponsah J . Diagnostic performance of an ultrasensitive HRP2-based malaria rapid diagnostic test kit used in surveys of afebrile people living in Southern Ghana. Malar J. 2021; 20(1):125. PMC: 7927401. DOI: 10.1186/s12936-021-03665-7. View

18.

Loiseau C, Cooper M, Doolan D . Deciphering host immunity to malaria using systems immunology. Immunol Rev. 2019; 293(1):115-143. DOI: 10.1111/imr.12814. View

19.

Aka K, Traore D, Sagna A, Zoh D, Assi S, Tchiekoi B . Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites. Malar J. 2020; 19(1):83. PMC: 7033907. DOI: 10.1186/s12936-020-03160-5. View

20.

Amoah B, McCann R, Kabaghe A, Mburu M, Chipeta M, Moraga P . Identifying transmission patterns through parasite prevalence and entomological inoculation rate. Elife. 2021; 10. PMC: 8530514. DOI: 10.7554/eLife.65682. View