Influence of the Solvent on the Stability of Aminopurine Tautomers and Properties of the Amino Group

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Apr 13

PMID 37049758

Authors

Anna Jezuita

Pawel A Wieczorkiewicz

Tadeusz M Krygowski

Halina Szatylowicz

Affiliations

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Abstract

Amino derivatives of purine (2-, 6-, 8-, and N-NH) have found many applications in biochemistry. This paper presents the results of a systematic computational study of the substituent and solvent effects in these systems. The issues considered are the electron-donating properties of NH, its geometry, π-electron delocalization in purine rings and tautomeric stability. Calculations were performed in ten environments, with 1 < ε < 109, using the polarizable continuum model of solvation. Electron-donating properties were quantitatively described by cSAR (charge of the substituent active region) parameter and π-electron delocalization by using the HOMA (harmonic oscillator model of aromaticity) index. In aminopurines, NH proximity interactions depend on its position and the tautomer. The results show that they are the main factor determining how solvation affects the electron-donating strength and geometry of NH. Proximity with the NH∙∙∙HN repulsive interaction between the NH and endocyclic NH group results in stronger solvent effects than the proximity with two attractive NH∙∙∙N interactions. The effect of amino and nitro (previously studied) substitution on aromaticity was compared; these two groups have, in most cases, the opposite effect, with the largest being in N1H and N3H purine tautomers. The amino group has a smaller effect on the tautomeric preferences of purine than the nitro group. Only in 8-aminopurine do tautomeric preferences change: N7H is more stable than N9H in HO.

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