Evaluation of Selenomethionine Entrapped in Nanoparticles for Oral Supplementation Using In Vitro, Ex Vivo and In Vivo Models

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Apr 13

PMID 37049704

Authors

Shane Forde

Giulianna Vozza

David J Brayden

Hugh J Byrne

Jesus M Frias

Sinead M Ryan

Affiliations

Soon will be listed here.

Abstract

Selenium methionine (SeMet) is an essential micronutrient required for normal body function and is associated with additional health benefits. However, oral administration of SeMet can be challenging due to its purported narrow therapeutic index, low oral bioavailability, and high susceptibility to oxidation. To address these issues, SeMet was entrapped in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The high stability of both the SeMet and selenomethionine nanoparticles (SeMet-NPs) was established using cultured human intestinal and liver epithelial cells, rat liver homogenates, and rat intestinal homogenates and lumen washes. Minimal cytotoxicity to Caco-2 and HepG2 cells was observed for SeMet and SeMet-NPs. Antioxidant properties of SeMet were revealed using a Reactive Oxygen Species (ROS) assay, based on the observation of a concentration-dependent reduction in the build-up of peroxides, hydroxides and hydroxyl radicals in Caco-2 cells exposed to SeMet (6.25-100 μM). The basal apparent permeability coefficient (P) of SeMet across isolated rat jejunal mucosae mounted in Ussing chambers was low, but the P was increased when presented in NP. SeMet had minimal effects on the electrogenic ion secretion of rat jejunal and colonic mucosae in Ussing chambers. Intra-jejunal injections of SeMet-NPs to rats yielded increased plasma levels of SeMet after 3 h for the SeMet-NPs compared to free SeMet. Overall, there is potential to further develop SeMet-NPs for oral supplementation due to the increased intestinal permeability, versus free SeMet, and the low potential for toxicity.

References

Dwipoerwantoro P, Lukito W, Aulia D, Arnaud J, Roussel A . Selenium status and fungi in the protein-losing enteropathy of persistent diarrhea. Asia Pac J Clin Nutr. 2017; 26(Suppl 1):S79-S84. DOI: 10.6133/apjcn.062017.s13. View

Cox A, Rawlinson L, Baird A, Bzik V, Brayden D . In vitro interactions between the oral absorption promoter, sodium caprate (C(10)) and S. typhimurium in rat intestinal ileal mucosae. Pharm Res. 2007; 25(1):114-22. DOI: 10.1007/s11095-007-9354-9. View

Matsukawa T, Hasegawa H, Goto H, Shinohara Y, Shinohara A, Omori Y . Evaluation of the metabolic chiral inversion of d-selenomethionine in rats by stable isotope dilution gas chromatography-mass spectrometry. J Pharm Biomed Anal. 2015; 116:59-64. DOI: 10.1016/j.jpba.2015.04.014. View

Vieira-Coelho M, Soares-da-Silva P . Ontogenic aspects of D1 receptor coupling to G proteins and regulation of rat jejunal Na+, K+ ATPase activity and electrolyte transport. Br J Pharmacol. 2000; 129(3):573-81. PMC: 1571852. DOI: 10.1038/sj.bjp.0703065. View

Calvo P, Remunan-Lopez C, Alonso M . Chitosan and chitosan/ethylene oxide-propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines. Pharm Res. 1997; 14(10):1431-6. DOI: 10.1023/a:1012128907225. View

Gutierrez-Bedmar M, Gil F, Olmedo P, Ruiz-Canela M, Martinez-Gonzalez M, Salas-Salvado J . Serum Selenium and Incident Cardiovascular Disease in the PREvención con DIeta MEDiterránea (PREDIMED) Trial: Nested Case-Control Study. J Clin Med. 2022; 11(22). PMC: 9692990. DOI: 10.3390/jcm11226664. View

Kucera O, Endlicher R, Rousar T, Lotkova H, Garnol T, Drahota Z . The effect of tert-butyl hydroperoxide-induced oxidative stress on lean and steatotic rat hepatocytes in vitro. Oxid Med Cell Longev. 2014; 2014:752506. PMC: 4009166. DOI: 10.1155/2014/752506. View

Kokarnig S, Tsirigotaki A, Wiesenhofer T, Lackner V, Francesconi K, Pergantis S . Concurrent quantitative HPLC-mass spectrometry profiling of small selenium species in human serum and urine after ingestion of selenium supplements. J Trace Elem Med Biol. 2014; 29:83-90. DOI: 10.1016/j.jtemb.2014.06.012. View

Cuthbert A, Margolius H . Kinins stimulate net chloride secretion by the rat colon. Br J Pharmacol. 1982; 75(4):587-98. PMC: 2071531. DOI: 10.1111/j.1476-5381.1982.tb09178.x. View

10.

Vozza G, Danish M, Byrne H, Frias J, Ryan S . Application of Box-Behnken experimental design for the formulation and optimisation of selenomethionine-loaded chitosan nanoparticles coated with zein for oral delivery. Int J Pharm. 2018; 551(1-2):257-269. DOI: 10.1016/j.ijpharm.2018.08.050. View

11.

Zhang H, Huang Q, Huang Z, Liu T, Li Y . Preparation and physicochemical properties of chitosan broadleaf holly leaf nanoparticles. Int J Pharm. 2014; 479(1):212-8. DOI: 10.1016/j.ijpharm.2014.12.010. View

12.

Xu H, Cao W, Zhang X . Selenium-containing polymers: promising biomaterials for controlled release and enzyme mimics. Acc Chem Res. 2013; 46(7):1647-58. DOI: 10.1021/ar4000339. View

13.

Aderao G, Jadhav S, Pattanaik A, Gupta S, Ramakrishnan S, Lokesha E . Dietary selenium levels modulates antioxidant, cytokine and immune response and selenoproteins mRNA expression in rats under heat stress condition. J Trace Elem Med Biol. 2022; 75:127105. DOI: 10.1016/j.jtemb.2022.127105. View

14.

Takahashi K, Suzuki N, Ogra Y . Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo. Int J Mol Sci. 2017; 18(3). PMC: 5372522. DOI: 10.3390/ijms18030506. View

15.

Aguirre T, Rosa M, Guterres S, Pohlmann A, Coulter I, Brayden D . Investigation of coco-glucoside as a novel intestinal permeation enhancer in rat models. Eur J Pharm Biopharm. 2014; 88(3):856-65. DOI: 10.1016/j.ejpb.2014.10.013. View

16.

Yoshida S, Iwataka M, Fuchigami T, Haratake M, Nakayama M . In vitro assessment of bioavailability of selenium from a processed Japanese anchovy, Niboshi. Food Chem. 2018; 269:436-441. DOI: 10.1016/j.foodchem.2018.07.033. View

17.

Zhang Z, Zhang Q, Li M, Xu J, Wang J, Li M . SeMet attenuates AFB1-induced intestinal injury in rabbits by activating the Nrf2 pathway. Ecotoxicol Environ Saf. 2022; 239:113640. DOI: 10.1016/j.ecoenv.2022.113640. View

18.

Muecke R, Schomburg L, Buentzel J, Kisters K, Micke O . Selenium or no selenium--that is the question in tumor patients: a new controversy. Integr Cancer Ther. 2010; 9(2):136-41. DOI: 10.1177/1534735410367648. View

19.

Kuhn F, Dorneles M, Norena C . Accelerated stability testing and simulated gastrointestinal release of encapsulated betacyanins and phenolic compounds from Bougainvillea glabra bracts extract. Food Chem. 2022; 393:133391. DOI: 10.1016/j.foodchem.2022.133391. View

20.

Sardou H, Akhgari A, Mohammadpour A, Namdar A, Kamali H, Jafarian A . Optimization study of combined enteric and time-dependent polymethacrylates as a coating for colon targeted delivery of 5-ASA pellets in rats with ulcerative colitis. Eur J Pharm Sci. 2021; 168:106072. DOI: 10.1016/j.ejps.2021.106072. View