PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating in Pomegranate

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Apr 13

PMID 37047337

Authors

Zenghui Wang

Xuemei Yang

Chuanzeng Wang

Lijuan Feng

Yanlei Yin

Jialin Li

Affiliations

Soon will be listed here.

Abstract

The peel color of pomegranates is an important exterior quality that determines market value. Anthocyanins are biosynthesized in the cytosol and then transported to the vacuole for storage. However, the molecular mechanism that determines the color variation between red and white pomegranates remains unclear. In this study, we identified an R2R3-MYB protein (PgMYB1) that interacts with the promoter and regulates its transcriptional expression, thus promoting the accumulation of anthocyanins in pomegranate. The expression of and was positively correlated with the anthocyanin content in red and white pomegranates. Further investigation showed that the knockdown of in red pomegranate 'Taishanhong' (TSH), by the virus-induced gene-silencing system, inhibited anthocyanin accumulation. Together, our results indicate that PgMYB1 controls the transport of anthocyanin via and thus promotes anthocyanin accumulation in red pomegranates. Our results have a certain reference value for further clarifying the regulation of anthocyanin synthesis and transport in pomegranate fruits.

References

Cardoso S, Lau W, Eiras Dias J, Fevereiro P, Maniatis N . A candidate-gene association study for berry colour and anthocyanin content in Vitis vinifera L. PLoS One. 2012; 7(9):e46021. PMC: 3461038. DOI: 10.1371/journal.pone.0046021. View

Larsen E, Alfenito M, Briggs W, Walbot V . A carnation anthocyanin mutant is complemented by the glutathione S-transferases encoded by maize Bz2 and petunia An9. Plant Cell Rep. 2003; 21(9):900-4. DOI: 10.1007/s00299-002-0545-x. View

Gomez C, Terrier N, Torregrosa L, Vialet S, Fournier-Level A, Verries C . Grapevine MATE-type proteins act as vacuolar H+-dependent acylated anthocyanin transporters. Plant Physiol. 2009; 150(1):402-15. PMC: 2675721. DOI: 10.1104/pp.109.135624. View

Alfenito M, Souer E, Goodman C, BUELL R, Mol J, Koes R . Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases. Plant Cell. 1998; 10(7):1135-49. PMC: 144053. DOI: 10.1105/tpc.10.7.1135. View

Francisco R, Regalado A, Ageorges A, Burla B, Bassin B, Eisenach C . ABCC1, an ATP binding cassette protein from grape berry, transports anthocyanidin 3-O-Glucosides. Plant Cell. 2013; 25(5):1840-54. PMC: 3694709. DOI: 10.1105/tpc.112.102152. View

Yuan Z, Yin Y, Qu J, Zhu L, Li Y . Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. J Genet Genomics. 2007; 34(12):1061-71. DOI: 10.1016/S1673-8527(07)60121-0. View

Lallement P, Brouwer B, Keech O, Hecker A, Rouhier N . The still mysterious roles of cysteine-containing glutathione transferases in plants. Front Pharmacol. 2014; 5:192. PMC: 4138524. DOI: 10.3389/fphar.2014.00192. View

Takos A, Jaffe F, Jacob S, Bogs J, Robinson S, Walker A . Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol. 2006; 142(3):1216-32. PMC: 1630764. DOI: 10.1104/pp.106.088104. View

Liu Y, Han X, Ren L, Yang H, Zeng Q . Functional divergence of the glutathione S-transferase supergene family in Physcomitrella patens reveals complex patterns of large gene family evolution in land plants. Plant Physiol. 2012; 161(2):773-86. PMC: 3561018. DOI: 10.1104/pp.112.205815. View

10.

Li J, Luan Q, Han J, Zhang C, Liu M, Ren Z . CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber. Hortic Res. 2020; 7:103. PMC: 7327083. DOI: 10.1038/s41438-020-0327-z. View

11.

Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T . Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol. 2007; 48(7):958-70. DOI: 10.1093/pcp/pcm066. View

12.

Hu B, Zhao J, Lai B, Qin Y, Wang H, Hu G . LcGST4 is an anthocyanin-related glutathione S-transferase gene in Litchi chinensis Sonn. Plant Cell Rep. 2016; 35(4):831-43. DOI: 10.1007/s00299-015-1924-4. View

13.

El-Sharkawy I, Liang D, Xu K . Transcriptome analysis of an apple (Malus × domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation. J Exp Bot. 2015; 66(22):7359-76. PMC: 4765799. DOI: 10.1093/jxb/erv433. View

14.

An J, Wang X, Li Y, Song L, Zhao L, You C . EIN3-LIKE1, MYB1, and ETHYLENE RESPONSE FACTOR3 Act in a Regulatory Loop That Synergistically Modulates Ethylene Biosynthesis and Anthocyanin Accumulation. Plant Physiol. 2018; 178(2):808-823. PMC: 6181056. DOI: 10.1104/pp.18.00068. View

15.

Edwards R, Dixon D . Plant glutathione transferases. Methods Enzymol. 2006; 401:169-86. DOI: 10.1016/S0076-6879(05)01011-6. View

16.

Wang Z, Li J, Yang X, Hu Y, Yin Y, Shen X . MdFLP enhances drought tolerance by regulating MdNAC019 in self-rooted apple stocks. Plant Sci. 2022; 321:111331. DOI: 10.1016/j.plantsci.2022.111331. View

17.

Jain M, Ghanashyam C, Bhattacharjee A . Comprehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses. BMC Genomics. 2010; 11:73. PMC: 2825235. DOI: 10.1186/1471-2164-11-73. View

18.

Wang Z, Li J, Mao Y, Zhang M, Wang R, Hu Y . Transcriptional regulation of MdPIN3 and MdPIN10 by MdFLP during apple self-rooted stock adventitious root gravitropism. BMC Plant Biol. 2019; 19(1):229. PMC: 6543673. DOI: 10.1186/s12870-019-1847-2. View

19.

Feild T, Lee D, Holbrook N . Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood. Plant Physiol. 2001; 127(2):566-74. PMC: 125091. View

20.

Jiang S, Chen M, He N, Chen X, Wang N, Sun Q . MdGSTF6, activated by MdMYB1, plays an essential role in anthocyanin accumulation in apple. Hortic Res. 2019; 6:40. PMC: 6395711. DOI: 10.1038/s41438-019-0118-6. View