Correlation of Redondovirus and Detections in the Human Oral Cavity Suggests That This Amoeba Is Possibly the Redondovirus Host

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Apr 13

PMID 37047275

Authors

Marine Makoa-Meng

Rayan Semmar

Angeline Antezack

Gwilherm Penant

Bernard La Scola

Virginie Monnet-Corti

Philippe Colson

Affiliations

Soon will be listed here.

Abstract

The virome of the human oral cavity and the relationships between viruses and diseases such as periodontitis are scarcely deciphered. Redondoviruses were reported in the human oral cavity in 2019, including in periodontitis patients. Here, we aimed at detecting redondoviruses and at searching for a potential viral host in human saliva. Non-stimulated saliva was collected between December 2020 and June 2021. These samples were tested using real-time PCR regarding the presence of redondovirus and DNA. Similarity searches were performed using BLAST against eukaryotic and prokaryotic sequences from GenBank. The redondovirus DNA was detected in 46% of the 28 human saliva samples. In addition, short fragments of redondovirus genomes were detected in silico within sequences. Finally, DNA was detected in 46% of the 28 saliva samples, with a strong correlation between redondovirus DNA and DNA detections, in 93% of the cases. Regarded together, these findings and previous ones strongly support the presence of redondoviruses in the human oral cavity and their association to as their likely host.

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