Complete Genome Sequence of Zoysia Mosaic Virus, a Novel Member of the Genus Poacevirus

Overview

Journal Arch Virol

Specialty Microbiology

Date 2023 Apr 12

PMID 37043050

Authors

Bishwo N Adhikari

Jing Zhou

Xiaojun Hu

Roy S Turner

Clint McFarland

Joseph A Foster

Affiliations

Soon will be listed here.

Abstract

Here, we report the detection and characterization of the genome of a novel poacevirus isolated from Zoysia matrella (Merrill) imported into the United States from Japan. The novel virus, tentatively named "zoysia mosaic virus" (ZoMV), is a single-stranded RNA virus with a genome of 9,728 nucleotides (nt) in length, encoding a large putative polyprotein of 3,119 amino acids (aa). The ZoMV genome is closely related to the triticum mosaic virus (TriMV; FJ263671) genome, with 57.18% nt and 51.74% aa sequence identity in the polyprotein region. Moreover, phylogenetic analysis showed that ZoMV is closely related to all other members of the genus Poacevirus. A survey of imported grasses showed that ZoMV was detected only in zoysiagrass. This is the first report of the complete genome sequence of a novel viral pathogen of zoysiagrass of the genus Poacevirus, for which we propose the binomial species name "Poacevirus zoisiae".

References

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

Buchfink B, Xie C, Huson D . Fast and sensitive protein alignment using DIAMOND. Nat Methods. 2014; 12(1):59-60. DOI: 10.1038/nmeth.3176. View

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

Fellers J, Seifers D, Ryba-White M, Martin T . The complete genome sequence of Triticum mosaic virus, a new wheat-infecting virus of the High Plains. Arch Virol. 2009; 154(9):1511-5. DOI: 10.1007/s00705-009-0462-1. View