Sp. Nov., Sp. Nov. and Sp. Nov., Isolated from Faeces from Qinghai-Tibet Plateau

Six aerobic or facultative anaerobic, motile, Gram-stain-positive, catalase-positive and oxidase-negative strains (zg-Y453, zg-Y324, zg-Y462, zg-Y411, zg-Y809 and zg-Y786) were isolated from different faecal samples of from the Qinghai-Tibet Plateau. Pale yellow, round, raised and moist colonies appeared 48 h after incubation at 28 °C on brain-heart infusion plates supplemented with 5 % defibrinated sheep blood. According to the 16S rRNA gene sequence alignment, two strain pairs (zg-Y453/zg-Y324 and zg-Y462/zg-Y411) shared the highest similarities to (99.5 and 99.2 %), and the other one (zg-Y809/zg-Y786) to (99.5 %). Results of phylogenetic analysis based on the 16S rRNA gene and genome sequences showed that these six strains represented three separate species within the genus . The average nucleotide identity and digital DNA-DNA hybridization values between the three novel type strains (zg-Y453/zg-Y462/zg-Y809) and other known species in this genus were all below respective thresholds (70.2-81.5/19.6-24.2 %, 70.6-81.8/19.8-25.0 %, and 70.4-88.2/19.9-35.3 %). Although phylogenetically related, there were obvious chemotaxonomic and phenotypic differences: strain pair zg-Y462/zg-Y411 had C as the only major fatty acid; the three novel species had different dominant quinones, MK-8(H) in strains zg-Y462/zg-Y809 (74.8/81.1 %) and MK-8(H)/MK-9(H) (43.1/53.0 %) in zg-Y453; similarly, the ability to reduce nitrate in strains zg-Y453 and zg-Y462 could differentiate them from zg-Y809. All strains had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, but differed slightly in the types of unidentified glycolipids, phospholipids and lipids. Based on the results of these polyphasic taxonomic analyses, three novel species within the genus are proposed, namely sp. nov. (type strain, zg-Y453=GDMCC 1.2809=JCM 35173), sp. nov. (type strain, zg-Y462=GDMCC 1.2880=JCM 35170) and sp. nov. (type strain, zg-Y809=GDMCC 1.2808=JCM 35168).