Determination of Immune Factor Levels in Serum and Local Hematoma Samples of Osteoporotic Fracture Patients and Clinical Study of the Effect of Active Vitamin D3 Treatment on Immune Factor Levels

Overview

Journal J Orthop Surg Res

Publisher Biomed Central

Specialty Orthopedics

Date 2023 Apr 10

PMID 37038178

Authors

Sijia Liu

Jianjun Li

Mingwei Zhang

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to investigate changes in systemic and local immune factors, namely, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, in patients with and without osteoporotic fractures and to explore the effects of active vitamin D3 treatment on immune function and fracture prognosis in patients with osteoporotic fractures.

Method: The mRNA expression levels of IL-1β, IL-6 and TNF-α were measured before the operation. After the operation, the patients in the control group were treated with conventional fracture treatment and calcium supplementation, and the patients in the treatment group were treated with calcium plus active vitamin D3 in addition to conventional fracture treatment. The serum of each patient was collected on the seventh day after the operation.

Results: The expression levels of the three immune factors (IL-1β, IL-6 and TNF-α) in the fracture end hematoma samples were significantly positively correlated with those in the serum samples (P < 0.05). The mean values of the serums of IL-1β, IL-6 and TNF-α in the osteoporosis group were significantly higher than those in the non-osteoporosis group (P < 0.05). The average number of hematomas in the osteoporosis group was significantly higher than that in the non-osteoporosis group (P < 0.05). The results for the active vitamin D3 treatment group were significantly lower than those for the control group (P < 0.05). The mean wrist function score of the active vitamin D3 treatment group was significantly better than that of the control group (P < 0.05). The average fracture healing time of the treatment group was significantly shorter than that of the control group (P < 0.05).

Conclusion: The relative expression of IL-1β, IL-6, and TNF-α in the fracture end hematoma samples was positively correlated with the corresponding levels of these immune factors in the serum samples. The levels of IL-1β, IL-6 and TNF-α in the serum and fracture end hematoma samples of the osteoporotic fracture patients were higher than those of the non-osteoporotic fracture patients. Active vitamin D3 treatment promoted fracture healing by affecting the levels of these immune factors.

Citing Articles

[Perioperative changes of serum interleukin 6 levels in elderly male patients with intertrochanteric fracture].

Chen X, Zhu H, Wu W, Tian C, Shi L, Fan W Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2024; 38(10):1249-1253.

PMID: 39433500 PMC: 11522526. DOI: 10.7507/1002-1892.202405057.

Systemic Inflammatory Regulators Associated with Osteoporosis: A Bidirectional Mendelian Randomization Study.

Xu L, Li H, Liu B, Han X, Sun H Calcif Tissue Int. 2024; 114(5):490-501.

PMID: 38528199 DOI: 10.1007/s00223-024-01200-9.

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