TAF15 Promotes Cell Proliferation, Migration and Invasion of Gastric Cancer Via Activation of the RAF1/MEK/ERK Signalling Pathway

Overview

Journal Sci Rep

Specialty Science

Date 2023 Apr 10

PMID 37037864

Authors

Li Tang

Chengming Guo

Xu Li

Bo Zhang

Liuye Huang

Affiliations

Soon will be listed here.

Abstract

TATA-box-binding protein-associated Factor 15 (TAF15), a member of the FUS/EWS/TAF15 (FET) family, contributes to the progression of various tumours. However, the role and molecular mechanism of TAF15 in gastric cancer (GC) progression are still unknown. In this study, we found that TAF15 was significantly upregulated in GC tumour tissues and cell lines. Overexpression of TAF15 was associated with a larger tumour size, high pathologic stage and high T stage of GC. TAF15 knockdown suppressed the proliferation, migration and invasion of GC cells in vitro and inhibited the tumour growth in vivo. Additionally, TAF15 knockdown led to the significant reductions in the phosphorylation levels of RAF1, MEK and ERK1/2, while total RAF1, MEK and ERK1/2 exhibited no significant change in GC cell lines. In summary, TAF15 is overexpressed in GC tumour tissues and cell lines, and promotes cell proliferation, migration and invasion in GC via the RAF1/MEK/ERK signaling pathway, which suggests that TAF15 might be a potential molecular diagnostic marker or therapeutic target for GC.

Citing Articles

Long noncoding RNA LINC01106 promotes lung adenocarcinoma progression via upregulation of autophagy.

Sun G, Zheng Y, Cai J, Gao J, Dong L, Zhang X Oncol Res. 2024; 33(1):171-184.

PMID: 39735666 PMC: 11671404. DOI: 10.32604/or.2024.047626.

[Sphingosine kinase-1 regulates migration and invasion of gastric cancer cells targeting the nuclear factor-κB signaling pathway].

Ling Q, Ji K, Chen J, Guan J, Wang R, Man W Nan Fang Yi Ke Da Xue Xue Bao. 2024; 44(11):2163-2171.

PMID: 39623272 PMC: 11605210. DOI: 10.12122/j.issn.1673-4254.2024.11.13.

CypA/TAF15/STAT5A/miR-514a-3p feedback loop drives ovarian cancer metastasis.

Li Y, Yang H, Li A, Chen B, Wang Y, Song Z Oncogene. 2024; 43(49):3570-3585.

PMID: 39402372 DOI: 10.1038/s41388-024-03188-w.

Tata-box-binding protein-associated factor 15 as a new potential marker in gastrointestinal tumors.

Elpek G World J Gastroenterol. 2024; 30(28):3367-3372.

PMID: 39091718 PMC: 11290397. DOI: 10.3748/wjg.v30.i28.3367.

Identification ATP5F1D as a Biomarker Linked to Diagnosis, Prognosis, and Immune Infiltration in Endometrial Cancer Based on Data-Independent Acquisition (DIA) Analysis.

Cheng Y, Liang X, Bi X, Liu C, Yang Y Biochem Genet. 2024; 62(5):4215-4236.

PMID: 38265620 DOI: 10.1007/s10528-023-10646-9.

References

Sasako M . Optimizing adjuvant therapies for the treatment of gastric cancer: with a special focus on Asia. Expert Rev Anticancer Ther. 2019; 19(11):939-945. DOI: 10.1080/14737140.2019.1685877. View

Zakaryan R, Gehring H . Identification and characterization of the nuclear localization/retention signal in the EWS proto-oncoprotein. J Mol Biol. 2006; 363(1):27-38. DOI: 10.1016/j.jmb.2006.08.018. View

Ullah R, Yin Q, Snell A, Wan L . RAF-MEK-ERK pathway in cancer evolution and treatment. Semin Cancer Biol. 2021; 85:123-154. DOI: 10.1016/j.semcancer.2021.05.010. View

Marko M, Vlassis A, Guialis A, Leichter M . Domains involved in TAF15 subcellular localisation: dependence on cell type and ongoing transcription. Gene. 2012; 506(2):331-8. DOI: 10.1016/j.gene.2012.06.088. View

Wang C, Ren C, Hu Q, Shen X, Wang M, Yang Z . Histidine-rich calcium binding protein promotes gastric cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition through Raf/MEK/ERK signaling. J Cancer. 2022; 13(4):1073-1085. PMC: 8899383. DOI: 10.7150/jca.68403. View

Blum A, Wang P, Zenklusen J . SnapShot: TCGA-Analyzed Tumors. Cell. 2018; 173(2):530. DOI: 10.1016/j.cell.2018.03.059. View

Schatz N, Brandlein S, Ruckl K, Hensel F, Vollmers H . Diagnostic and therapeutic potential of a human antibody cloned from a cancer patient that binds to a tumor-specific variant of transcription factor TAF15. Cancer Res. 2010; 70(1):398-408. DOI: 10.1158/0008-5472.CAN-09-2186. View

Smyth E, Nilsson M, Grabsch H, van Grieken N, Lordick F . Gastric cancer. Lancet. 2020; 396(10251):635-648. DOI: 10.1016/S0140-6736(20)31288-5. View

Su Z, Sun Z, Wang Z, Wang S, Wang Y, Jin E . TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex. Cell Rep. 2022; 41(3):111513. DOI: 10.1016/j.celrep.2022.111513. View

10.

Peng W, He P, Liu L, Zhu T, Zhong Y, Xiang L . LncRNA GAS5 activates the HIF1A/VEGF pathway by binding to TAF15 to promote wound healing in diabetic foot ulcers. Lab Invest. 2021; 101(8):1071-1083. DOI: 10.1038/s41374-021-00598-2. View

11.

Kang W, Tong J, Chan A, Lee T, Lung R, Leung P . Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis. Clin Cancer Res. 2011; 17(8):2130-9. DOI: 10.1158/1078-0432.CCR-10-2467. View

12.

Cao W, Chen H, Yu Y, Li N, Chen W . Changing profiles of cancer burden worldwide and in China: a secondary analysis of the global cancer statistics 2020. Chin Med J (Engl). 2021; 134(7):783-791. PMC: 8104205. DOI: 10.1097/CM9.0000000000001474. View

13.

Vollmers H, Birchmeier W . Monoclonal antibodies inhibit the adhesion of mouse B 16 melanoma cells in vitro and block lung metastasis in vivo. Proc Natl Acad Sci U S A. 1983; 80(12):3729-33. PMC: 394124. DOI: 10.1073/pnas.80.12.3729. View

14.

Glisovic T, Bachorik J, Yong J, Dreyfuss G . RNA-binding proteins and post-transcriptional gene regulation. FEBS Lett. 2008; 582(14):1977-86. PMC: 2858862. DOI: 10.1016/j.febslet.2008.03.004. View

15.

Zhou Y, Li Z, Wu X, Tou L, Zheng J, Zhou D . MAGOH/MAGOHB Inhibits the Tumorigenesis of Gastric Cancer via Inactivation of b-RAF/MEK/ERK Signaling. Onco Targets Ther. 2020; 13:12723-12735. PMC: 7735944. DOI: 10.2147/OTT.S263913. View

16.

Linden M, Vannas C, Osterlund T, Andersson L, Osman A, Escobar M . FET fusion oncoproteins interact with BRD4 and SWI/SNF chromatin remodelling complex subtypes in sarcoma. Mol Oncol. 2022; 16(13):2470-2495. PMC: 9251840. DOI: 10.1002/1878-0261.13195. View

17.

Zuo L, Zhang G, Massett M, Cheng J, Guo Z, Wang L . Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription. Nat Commun. 2021; 12(1):1491. PMC: 7935978. DOI: 10.1038/s41467-021-21690-7. View

18.

Kume K, Ikeda M, Miura S, Ito K, Sato K, Ohmori Y . α-Amanitin Restrains Cancer Relapse from Drug-Tolerant Cell Subpopulations via TAF15. Sci Rep. 2016; 6:25895. PMC: 4867652. DOI: 10.1038/srep25895. View

19.

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

20.

Ruan X, Zheng J, Liu X, Liu Y, Liu L, Ma J . lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation. Mol Ther Nucleic Acids. 2020; 20:823-840. PMC: 7256440. DOI: 10.1016/j.omtn.2020.05.003. View