Establishment, Optimization, and Application of Genetic Technology in Spp

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Apr 7

PMID 37025635

Authors

Jing Gao

Huiqing Liu

Zhenzhen Zhang

Zhihong Liang

Affiliations

Soon will be listed here.

Abstract

is widely distributed in nature and occupies a crucial ecological niche, which has complex and diverse metabolic pathways and can produce a variety of metabolites. With the deepening of genomics exploration, more genomic informations have been elucidated, which not only help us understand the basic mechanism of various life activities, but also further realize the ideal functional transformation. Available genetic engineering tools include homologous recombinant systems, specific nuclease based systems, and RNA techniques, combined with transformation methods, and screening based on selective labeling. Precise editing of target genes can not only prevent and control the production of mycotoxin pollutants, but also realize the construction of economical and efficient fungal cell factories. This paper reviewed the establishment and optimization process of genome technologies, hoping to provide the theoretical basis of experiments, and summarized the recent progress and application in genetic technology, analyzes the challenges and the possibility of future development with regard to .

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