Application of Nanotags and Nanobodies for Live Cell Single-molecule Imaging of the Z-ring in Escherichia Coli

Overview

Journal Curr Genet

Specialty Genetics

Date 2023 Apr 6

PMID 37022498

Authors

Emma Westlund

Axel Bergenstrale

Alaska Pokhrel

Helena Chan

Ulf Skoglund

Daniel O Daley

Bill Soderstrom

Affiliations

Soon will be listed here.

Abstract

Understanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.

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