Using Display Technologies to Identify Macrocyclic Peptide Antibiotics

Overview

Journal Biochim Biophys Acta Mol Cell Res

Publisher Elsevier

Specialties Biochemistry
Biophysics
Cell Biology

Date 2023 Apr 3

PMID 37011732

Authors

Justin R Randall

Xun Wang

Kyra E Groover

Angela C ODonnell

Bryan W Davies

Affiliations

Soon will be listed here.

Abstract

Antibiotic resistant bacterial infections are now a leading cause of global mortality. While drug resistance continues to spread, the clinical antibiotic pipeline has become bare. This discord has focused attention on developing new strategies for antimicrobial discovery. Natural macrocyclic peptide-based products have provided novel antibiotics and antibiotic scaffolds targeting several essential bacterial cell envelope processes, but discovery of such natural products remains a slow and inefficient process. Synthetic strategies employing peptide display technologies can quickly screen large libraries of macrocyclic sequences for specific target binding and general antibacterial potential providing alternative approaches for new antibiotic discovery. Here we review cell envelope processes that can be targeted with macrocyclic peptide therapeutics, outline important macrocyclic peptide display technologies, and discuss future strategies for both library design and screening.

Citing Articles

Adapting antibacterial display to identify serum-active macrocyclic peptide antibiotics.

Randall J, Groover K, ODonnell A, Garza J, Cole T, Davies B PNAS Nexus. 2023; 2(8):pgad270.

PMID: 37637199 PMC: 10449418. DOI: 10.1093/pnasnexus/pgad270.

Adapting antibacterial display to identify serum active macrocyclic peptide antibiotics.

Randall J, Groover K, ODonnell A, Garza J, Cole T, Davies B bioRxiv. 2023; .

PMID: 37546850 PMC: 10402130. DOI: 10.1101/2023.07.28.550711.

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