Effect of Host Lex, RecA, RecF, and UvrD Genotypes on the Ultraviolet Light-protecting and Related Properties of Plasmid R46 in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1979 Feb 1

PMID 370103

Citations 15

Authors

N S Waleh

B A Stocker

Affiliations

Soon will be listed here.

Abstract

The ability of plasmid R46 to reduce the lethal but enhance the mutagenic effect of ultraviolet (UV) irradiation was tested in sets of Escherichia coli K-12 derivatives, wild type or with different mutations affecting DNA repair capacity, but otherwise isogenic. UV protection and enhancement of UV mutagenic effect were obtained in uvrA6, uvrB5, uvrD3, and recF143 hosts, but not in a recA56 strain. The plasmid gave some UV protection in two lexA1 and two lexA101 strains and in one lexA102 host, but produced no such effect in another lexA102 host. The plasmid restored UV mutagenic effect in a lexB30 strain, the yield of induced mutants per survivor of irradiation (10 J/m2) being about the same for the lexB30(R46) and lex+(R46) strains; by contrast the plasmid, though it reduced the UV sensitivity of the lexB30 strain, did not make it as UV-resistant as the lex+ R-strain.

Citing Articles

DNA Metabolism in Balance: Rapid Loss of a RecA-Based Hyperrec Phenotype.

Bakhlanova I, Dudkina A, Wood E, Lanzov V, Cox M, Baitin D PLoS One. 2016; 11(4):e0154137.

PMID: 27124470 PMC: 4849656. DOI: 10.1371/journal.pone.0154137.

Repair and plasmid R46 mediated mutation requires inducible functions in Proteus mirabilis.

Hofemeister J, Eitner G Mol Gen Genet. 1981; 183(2):369-75.

PMID: 7035831 DOI: 10.1007/BF00270642.

The muc+ gene of plasmid pKM101 prevents respiration shutoff in far ultraviolet-irradiated Salmonella typhimurium.

Swenson P Mol Gen Genet. 1981; 182(3):422-5.

PMID: 7029212 DOI: 10.1007/BF00293930.

Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

Walker G Microbiol Rev. 1984; 48(1):60-93.

PMID: 6371470 PMC: 373003. DOI: 10.1128/mr.48.1.60-93.1984.

The muc genes of pKM101 are induced by DNA damage.

Elledge S, Walker G J Bacteriol. 1983; 155(3):1306-15.

PMID: 6350267 PMC: 217829. DOI: 10.1128/jb.155.3.1306-1315.1983.

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