Expanding Structural Space for Immunomodulatory Nucleic Acid Nanoparticles (Nanps) Via Spatial Arrangement of Their Therapeutic Moieties

Overview

Journal Adv Funct Mater

Date 2023 Apr 3

PMID 37008199

Authors

Morgan Chandler

Lewis Rolband

M Brittany Johnson

Da Shi

Yelixza I Avila

Edward Cedrone

Damian Beasock

Leyla Danai

Elizabeth Stassenko

Joanna K Krueger

Jiancheng Jiang

Jeoung Soo Lee

Marina A Dobrovolskaia

Kirill A Afonin

Affiliations

Soon will be listed here.

Abstract

Different therapeutic nucleic acids (TNAs) can be unified in a single structure by their elongation with short oligonucleotides designed to self-assemble into nucleic acid nanoparticles (NANPs). With this approach, therapeutic cocktails with precisely controlled composition and stoichiometry of active ingredients can be delivered to the same diseased cells for enhancing pharmaceutical action. In this work, an additional nanotechnology-based therapeutic option that enlists a biocompatible NANP-encoded platform for their controlled patient-specific immunorecognition is explored. For this, a set of representative functional NANPs is extensively characterized in vitro, ex vivo, and in vivo and then further analyzed for immunostimulation of human peripheral blood mononuclear cells freshly collected from healthy donor volunteers. The results of the study present the advancement of the current TNA approach toward personalized medicine and offer a new strategy to potentially address top public health challenges related to drug overdose and safety through the biodegradable nature of the functional platform with immunostimulatory regulation.

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