Separation of Cryptosporidium Oocysts from Fecal Debris by Density Gradient Centrifugation and Glass Bead Columns

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1986 Apr 1

PMID 3700630

Citations 9

Authors

M B Heyman

L K Shigekuni

A J Ammann

Affiliations

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Abstract

A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.

Citing Articles

Chromosome-level genome assembly of Cryptosporidium parvum by long-read sequencing of ten oocysts.

Chen Y, Huang J, Qin H, Zhang K, Fu Y, Li J Sci Data. 2024; 11(1):1287.

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Revisiting the infectivity and pathogenicity of Cryptosporidium avium provides new information on parasitic sites within the host.

Cui Z, Song D, Qi M, Zhang S, Wang R, Jian F Parasit Vectors. 2018; 11(1):514.

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Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice.

Del Coco V, Sparo M, Sidoti A, Santin M, Basualdo J, Cordoba M Parasitol Res. 2016; 115(8):3239-44.

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DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR.

Hawash Y Korean J Parasitol. 2014; 52(3):263-71.

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Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces.

Suresh P, Rehg J J Clin Microbiol. 1996; 34(1):38-40.

PMID: 8748268 PMC: 228725. DOI: 10.1128/jcm.34.1.38-40.1996.

References

Wagenbach G . Purification of Eimeria tenella sporozoites with glass bead columns. J Parasitol. 1969; 55(4):833-8. View

Sherwood D, Angus K, Snodgrass D, Tzipori S . Experimental cryptosporidiosis in laboratory mice. Infect Immun. 1982; 38(2):471-5. PMC: 347763. DOI: 10.1128/iai.38.2.471-475.1982. View

Sharma N, REID W, FOSTER J . A cleaning method for coccidial oocysts using density-gradient sedimentation. J Parasitol. 1963; 49:159-60. View

Upton S, Current W . The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. J Parasitol. 1985; 71(5):625-9. View

MARQUARDT W . Separation of nematode eggs from fecal debris by gradient centrifugation. J Parasitol. 1961; 47:248-50. View