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Serodiagnosis of La Crosse Virus Infections in Humans by Detection of Immunoglobulin M Class Antibodies

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1986 Apr 1
PMID 3700625
Citations 9
Authors
C H Calisher
C I Pretzman
D J Muth
M A Parsons
E D Peterson
Affiliations
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Abstract

Sera from 92 humans with illnesses clinically compatible with those caused by California serogroup virus infections were tested for antibody to La Crosse (LAC) virus by using the immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC ELISA), the IgG ELISA, and the hemagglutination inhibition (HI), complement fixation and serum dilution-plaque reduction neutralization tests. On the reported day of onset of illness in 18 individuals, 94% had IgM antibody, 50% had neutralization antibody, 33% had HI antibody, and 11% had IgG antibody. Neutralization, HI, and IgG antibody prevalence rates increased thereafter, whereas IgM antibody prevalence remained high (92% 2 or more weeks after the onset of illness). It was concluded that the MAC ELISA is a sensitive test for the presence of antibody to LAC virus. The sensitivity of the MAC ELISA and the rapidity with which it can be performed appear to provide a powerful tool for the clinically relevant serodiagnosis of LAC virus infections in humans.

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References
1.
Deering W . Neurologic aspects and treatment of La Crosse encephalitis. Prog Clin Biol Res. 1983; 123:187-91. View
2.
Beaty B, Jamnback T, Hildreth S, Brown K . Rapid diagnosis of La Crosse virus infections: evaluation of serologic and antigen detection techniques for the clinically relevant diagnosis of La Crosse encephalitis. Prog Clin Biol Res. 1983; 123:293-302. View
3.
DEIBEL R, SRIHONGSE S, Grayson M, Grimstad P, MAHDY M, Artsob H . Jamestown Canyon virus: the etiologic agent of an emerging human disease?. Prog Clin Biol Res. 1983; 123:313-25. View
4.
Monath T, Nystrom R, Bailey R, Calisher C, Muth D . Immunoglobulin M antibody capture enzyme-linked immunosorbent assay for diagnosis of St. Louis encephalitis. J Clin Microbiol. 1984; 20(4):784-90. PMC: 271431. DOI: 10.1128/jcm.20.4.784-790.1984. View
5.
Calisher C, Travassos da Rosa A, Bartz C, BRUMMER-KORVENKONTIO M, Haksohusodo S, Suharyono W . Complex-specific immunoglobulin M antibody patterns in humans infected with alphaviruses. J Clin Microbiol. 1986; 23(1):155-9. PMC: 268591. DOI: 10.1128/jcm.23.1.155-159.1986. View