Spatial Subcellular Organelle Networks in Single Cells

Overview

Journal Sci Rep

Specialty Science

Date 2023 Apr 3

PMID 37005468

Authors

Mythreye Venkatesan

Nicholas Zhang

Benoit Marteau

Yukina Yajima

Nerea Ortiz De Zarate Garcia

Zhou Fang

Thomas Hu

Shuangyi Cai

Adam Ford

Harrison Olszewski

Andrew Borst

Ahmet F Coskun

Affiliations

Soon will be listed here.

Abstract

Organelles play important roles in human health and disease, such as maintaining homeostasis, regulating growth and aging, and generating energy. Organelle diversity in cells not only exists between cell types but also between individual cells. Therefore, studying the distribution of organelles at the single-cell level is important to understand cellular function. Mesenchymal stem cells are multipotent cells that have been explored as a therapeutic method for treating a variety of diseases. Studying how organelles are structured in these cells can answer questions about their characteristics and potential. Herein, rapid multiplexed immunofluorescence (RapMIF) was performed to understand the spatial organization of 10 organelle proteins and the interactions between them in the bone marrow (BM) and umbilical cord (UC) mesenchymal stem cells (MSCs). Spatial correlations, colocalization, clustering, statistical tests, texture, and morphological analyses were conducted at the single cell level, shedding light onto the interrelations between the organelles and comparisons of the two MSC subtypes. Such analytics toolsets indicated that UC MSCs exhibited higher organelle expression and spatially spread distribution of mitochondria accompanied by several other organelles compared to BM MSCs. This data-driven single-cell approach provided by rapid subcellular proteomic imaging enables personalized stem cell therapeutics.

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