Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash () Lines

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 Mar 30

PMID 36992495

Authors

Saritha Raman Kavalappara

Sudeep Bag

Alex Luckew

Cecilia E McGregor

Affiliations

Soon will be listed here.

Abstract

RNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in line PI 420328 with tolerance to cucurbit yellow stunting disorder virus (CYSDV) were compared with those in Gold Star, a susceptible cultivar. The lower CYSDV symptom severity in PI 420328 correlated with lower virus titers and fewer sRNAs derived from CYSDV (vsRNA) compared to Gold Star. Elevated levels of 21- and 22-nucleotide (nt) size class vsRNAs were observed in PI 420328, indicating more robust and efficient RNA silencing in PI 420328. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star. However, the 3' UTRs, CPm, and p26 were targeted at a higher frequency in PI 420328.

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References

Ogwok E, Ilyas M, Alicai T, Rey M, Taylor N . Comparative analysis of virus-derived small RNAs within cassava (Manihot esculenta Crantz) infected with cassava brown streak viruses. Virus Res. 2016; 215:1-11. PMC: 4796025. DOI: 10.1016/j.virusres.2016.01.015. View

Kavalappara S, Riley D, Cremonez P, Perier J, Bag S . Wild Radish ( L.) Is a Potential Reservoir Host of Cucurbit Chlorotic Yellows Virus. Viruses. 2022; 14(3). PMC: 8950442. DOI: 10.3390/v14030593. View

Mitter N, Worrall E, Robinson K, Li P, Jain R, Taochy C . Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses. Nat Plants. 2017; 3:16207. DOI: 10.1038/nplants.2016.207. View

Brodersen P, Voinnet O . The diversity of RNA silencing pathways in plants. Trends Genet. 2006; 22(5):268-80. DOI: 10.1016/j.tig.2006.03.003. View

Shidore T, Zuverza-Mena N, da Silva W . Small RNA profiling analysis of two recombinant strains of potato virus Y in infected tobacco plants. Virus Res. 2020; 288:198125. DOI: 10.1016/j.virusres.2020.198125. View

Carbonell A, Carrington J . Antiviral roles of plant ARGONAUTES. Curr Opin Plant Biol. 2015; 27:111-7. PMC: 4618181. DOI: 10.1016/j.pbi.2015.06.013. View

Herranz M, Navarro J, Sommen E, Pallas V . Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems. BMC Genomics. 2015; 16:117. PMC: 4345012. DOI: 10.1186/s12864-015-1327-5. View

Bouche N, Lauressergues D, Gasciolli V, Vaucheret H . An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs. EMBO J. 2006; 25(14):3347-56. PMC: 1523179. DOI: 10.1038/sj.emboj.7601217. View

Golyaev V, Candresse T, Rabenstein F, Pooggin M . Plant virome reconstruction and antiviral RNAi characterization by deep sequencing of small RNAs from dried leaves. Sci Rep. 2019; 9(1):19268. PMC: 6917709. DOI: 10.1038/s41598-019-55547-3. View

10.

Voinnet O . Post-transcriptional RNA silencing in plant-microbe interactions: a touch of robustness and versatility. Curr Opin Plant Biol. 2008; 11(4):464-70. DOI: 10.1016/j.pbi.2008.04.006. View

11.

Shahid S, Kim G, Johnson N, Wafula E, Wang F, Coruh C . MicroRNAs from the parasitic plant Cuscuta campestris target host messenger RNAs. Nature. 2018; 553(7686):82-85. DOI: 10.1038/nature25027. View

12.

Moyo L, Ramesh S, Kappagantu M, Mitter N, Sathuvalli V, Pappu H . The effects of potato virus Y-derived virus small interfering RNAs of three biologically distinct strains on potato (Solanum tuberosum) transcriptome. Virol J. 2017; 14(1):129. PMC: 5513076. DOI: 10.1186/s12985-017-0803-8. View

13.

Ding S, Voinnet O . Antiviral immunity directed by small RNAs. Cell. 2007; 130(3):413-26. PMC: 2703654. DOI: 10.1016/j.cell.2007.07.039. View

14.

Devers E, Brosnan C, Sarazin A, Albertini D, Amsler A, Brioudes F . Movement and differential consumption of short interfering RNA duplexes underlie mobile RNA interference. Nat Plants. 2020; 6(7):789-799. DOI: 10.1038/s41477-020-0687-2. View

15.

Liu S, Wang C, Liu X, Navas-Castillo J, Zang L, Fan Z . Tomato chlorosis virus-encoded p22 suppresses auxin signalling to promote infection via interference with SKP1-Cullin-F-box complex assembly. Plant Cell Environ. 2021; 44(9):3155-3172. DOI: 10.1111/pce.14125. View

16.

Voorburg C, Bai Y, Kormelink R . Small RNA Profiling of Susceptible and Resistant Encoding Tomato Plants Upon Tomato Yellow Leaf Curl Virus Infection. Front Plant Sci. 2021; 12:757165. PMC: 8637622. DOI: 10.3389/fpls.2021.757165. View

17.

Xia Z, Peng J, Li Y, Chen L, Li S, Zhou T . Characterization of small interfering RNAs derived from Sugarcane mosaic virus in infected maize plants by deep sequencing. PLoS One. 2014; 9(5):e97013. PMC: 4018358. DOI: 10.1371/journal.pone.0097013. View

18.

Tzanetakis I, Martin R, Wintermantel W . Epidemiology of criniviruses: an emerging problem in world agriculture. Front Microbiol. 2013; 4:119. PMC: 3656352. DOI: 10.3389/fmicb.2013.00119. View

19.

Silva T, Romanel E, Andrade R, Farinelli L, Osteras M, Deluen C . Profile of small interfering RNAs from cotton plants infected with the polerovirus Cotton leafroll dwarf virus. BMC Mol Biol. 2011; 12:40. PMC: 3189115. DOI: 10.1186/1471-2199-12-40. View

20.

Naveed K, Mitter N, Harper A, Dhingra A, Pappu H . Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solanum tuberosum) cv. Russet Burbank. Virus Res. 2014; 191:153-60. DOI: 10.1016/j.virusres.2014.07.005. View