Towards Non-target Proactive Food Safety: Identification of Active Compounds in Convenience Tomato Products by Ten-dimensional Hyphenation with Integrated Simulated Gastrointestinal Digestion

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2023 Mar 29

PMID 36988684

Authors

Tamara Schreiner

Naila M Eggerstorfer

Gertrud E Morlock

Affiliations

Soon will be listed here.

Abstract

Current strategies for non-target food screening focus mainly on known hazardous chemicals (adulterants, residues, contaminants, packaging migrants, etc.) instead of bioactive constituents in general and exclude the biological effect detection. To widen the perspective, a more proactive non-target effect-directed strategy is introduced to complement food safety in order to detect not only known but also unknown bioactive compounds. The developed 10-dimensional hyphenation included on-surface digestion (1D), planar chromatographic separation (2D), visualization using white light (3D), UV light (4D), fluorescence light (5D), effect-directed assay analysis (6D), heart-cut zone elution to an orthogonal reversed phase column chromatography including online desalting (7D) with subsequent diode array detection (8D), high-resolution mass spectrometry (9D), and fragmentation (10D). Metabolism, i.e., intestinal digestion of each sample, was simulated and integrated on the same adsorbent surface to study any changes in the compound profiles. As proof of principle, nine convenience tomato products and a freshly prepared tomato soup were screened via five different planar assays in a non-targeted mode. Non-digested and digested samples were compared side by side. In their effect-directed profiles, 14 bioactive compounds from classes of lipids, plant hormones, spices, and pesticides were identified. In particular, bioactive compounds coming from the lipid class were increased by gastrointestinal digestion, while spices and pesticides remained unaffected. With regard to food safety, the determination of the two dinitrophenol herbicides dinoterb and dinoseb in highly processed tomato products should be given special attention. The hyphenation covered a broad analyte spectrum and showed robust and reliable results.

References

Morlock G, Meyer D . Designed genotoxicity profiling detects genotoxic compounds in staple food such as healthy oils. Food Chem. 2022; 408:135253. DOI: 10.1016/j.foodchem.2022.135253. View

Haq I, Imran M, Nadeem M, Tufail T, Gondal T, Mubarak M . Piperine: A review of its biological effects. Phytother Res. 2020; 35(2):680-700. DOI: 10.1002/ptr.6855. View

Jia W, Shi L, Chu X, Chang J, Chen Y, Zhang F . A strategy for untargeted screening of macrolides and metabolites in bass by liquid chromatography coupled to quadrupole orbitrap mass spectrometry. Food Chem. 2018; 262:110-117. DOI: 10.1016/j.foodchem.2018.04.090. View

Kongstad K, Ozdemir C, Barzak A, Wubshet S, Staerk D . Combined use of high-resolution α-glucosidase inhibition profiling and high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for investigation of antidiabetic.... J Agric Food Chem. 2015; 63(8):2257-63. DOI: 10.1021/jf506297k. View

Schreiner T, Eggerstorfer N, Morlock G . Ten-dimensional hyphenation including simulated static gastro-intestinal digestion on the adsorbent surface, planar assays, and bioactivity evaluation for meal replacement products. Food Funct. 2022; 14(1):344-353. DOI: 10.1039/d2fo02610d. View

Salehi B, Tsouh Fokou P, Sharifi-Rad M, Zucca P, Pezzani R, Martins N . The Therapeutic Potential of Naringenin: A Review of Clinical Trials. Pharmaceuticals (Basel). 2019; 12(1). PMC: 6469163. DOI: 10.3390/ph12010011. View

Chanforan C, Loonis M, Mora N, Caris-Veyrat C, Dufour C . The impact of industrial processing on health-beneficial tomato microconstituents. Food Chem. 2013; 134(4):1786-95. DOI: 10.1016/j.foodchem.2012.03.077. View

Morlock G, Drotleff L, Brinkmann S . Miniaturized all-in-one nanoGIT system for on-surface metabolization, separation and effect imaging. Anal Chim Acta. 2021; 1154:338307. DOI: 10.1016/j.aca.2021.338307. View

Rodriguez-Roque M, Rojas-Grau M, Elez-Martinez P, Martin-Belloso O . Soymilk phenolic compounds, isoflavones and antioxidant activity as affected by in vitro gastrointestinal digestion. Food Chem. 2012; 136(1):206-12. DOI: 10.1016/j.foodchem.2012.07.115. View

10.

Schreiner T, Sauter D, Friz M, Heil J, Morlock G . Is Our Natural Food Our Homeostasis? Array of a Thousand Effect-Directed Profiles of 68 Herbs and Spices. Front Pharmacol. 2021; 12:755941. PMC: 8696259. DOI: 10.3389/fphar.2021.755941. View

11.

Sollano-Mendieta X, Meza-Marquez O, Osorio-Revilla G, Tellez-Medina D . Effect of In Vitro Digestion on the Antioxidant Compounds and Antioxidant Capacity of 12 Plum ( L.) Ecotypes. Foods. 2021; 10(9). PMC: 8471911. DOI: 10.3390/foods10091995. View

12.

Rusko J, Perkons I, Rasinger J, Bartkevics V . Non-target and suspected-target screening for potentially hazardous chemicals in food contact materials: investigation of paper straws. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020; 37(4):649-664. DOI: 10.1080/19440049.2020.1711969. View

13.

Schreiner T, Morlock G . Non-target bioanalytical eight-dimensional hyphenation including bioassay, heart-cut trapping, online desalting, orthogonal separations and mass spectrometry. J Chromatogr A. 2021; 1647:462154. DOI: 10.1016/j.chroma.2021.462154. View

14.

Gonzalez-Gaya B, Lopez-Herguedas N, Bilbao D, Mijangos L, Iker A, Etxebarria N . Suspect and non-target screening: the last frontier in environmental analysis. Anal Methods. 2021; 13(16):1876-1904. DOI: 10.1039/d1ay00111f. View

15.

Schreiner T, Ronzheimer A, Friz M, Morlock G . Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation. Food Chem. 2022; 395:133610. DOI: 10.1016/j.foodchem.2022.133610. View

16.

Wu I, Turnipseed S, Storey J, Andersen W, Madson M . Comparison of data acquisition modes with Orbitrap high-resolution mass spectrometry for targeted and non-targeted residue screening in aquacultured eel. Rapid Commun Mass Spectrom. 2019; 34(7):e8642. PMC: 7722469. DOI: 10.1002/rcm.8642. View

17.

Willems J, Khamis M, Mohammed Saeid W, Purves R, Katselis G, Low N . Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry. Anal Chim Acta. 2016; 933:164-74. DOI: 10.1016/j.aca.2016.05.041. View

18.

Boersma M, van der Woude H, Bogaards J, Boeren S, Vervoort J, Cnubben N . Regioselectivity of phase II metabolism of luteolin and quercetin by UDP-glucuronosyl transferases. Chem Res Toxicol. 2002; 15(5):662-70. DOI: 10.1021/tx0101705. View

19.

Costantini L, Molinari R, Farinon B, Merendino N . Impact of Omega-3 Fatty Acids on the Gut Microbiota. Int J Mol Sci. 2017; 18(12). PMC: 5751248. DOI: 10.3390/ijms18122645. View

20.

Qiao X, Ye M, Pan D, Miao W, Xiang C, Han J . Differentiation of various traditional Chinese medicines derived from animal bile and gallstone: simultaneous determination of bile acids by liquid chromatography coupled with triple quadrupole mass spectrometry. J Chromatogr A. 2010; 1218(1):107-17. DOI: 10.1016/j.chroma.2010.10.116. View