Identification and Expression Analysis of Hexokinases Family in L. Under Drought and Cold Stresses

Overview

Journal Plants (Basel)

Date 2023 Mar 29

PMID 36986904

Authors

Ying Liu

Yaolan Jiang

Xiaolan Liu

Hefen Cheng

Yuekun Han

Dawei Zhang

Jinfeng Wu

Lili Liu

Mingli Yan

Youxiong Que

Dinggang Zhou

Affiliations

Soon will be listed here.

Abstract

In plants, the multi-gene family of dual-function hexokinases (HXKs) plays an important role in sugar metabolism and sensing, that affects growth and stress adaptation. Sugarcane is an important sucrose crop and biofuel crop. However, little is known about the gene family in sugarcane. A comprehensive survey of sugarcane , including physicochemical properties, chromosomal distribution, conserved motifs, and gene structure was conducted, identifying 20 members of the gene family that were located on seven of the 32 L. chromosomes. Phylogenetic analysis showed that the family could be divided into three subfamilies (group I, II and III). Motifs and gene structure were related to the classification of . Most contained 8-11 introns which was consistent with other monocots. Duplication event analysis indicated that in L. primarily originated from segmental duplication. We also identified putative cis-elements in the promoter regions which were involved in phytohormone, light and abiotic stress responses (drought, cold et al.). During normal growth and development, 17 were constitutively expressed in all ten tissues. Among them, , and had similar expression patterns and were more highly expressed than other genes at all times. The RNA-seq analysis showed that 14/20 had the highest expression level after cold stress for 6 h, especially , and . As for drought treatment, 7/20 had the highest expression level after drought stress for 10 days, 3/20 (, and ) had the highest expression level after 10 days of recovery. Overall, our results revealed the potential biological function of , which may provide information for in-depth functional verification.

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