Production of Tetramethylpyrazine from Cane Molasses by Sp. TTMP20

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Mar 29

PMID 36985611

Authors

Yujia Li

Lirong Luo

Xiaoxiao Ding

Xiumin Zhang

Shanling Gan

Changhua Shang

Affiliations

Soon will be listed here.

Abstract

2,3,5,6-Tetramethylpyrazine (TTMP) is an active ingredient of Franch. It can be used in medicine and food fields. In this study, sp. TTMP20 was applied to produce TTMP using cane molasses as a carbon source. After pretreatment with phosphoric acid, 170 mL/L treated molasses, combined with 10 g/L yeast powder, 30 g/L tryptone and 30 g/L (NH)HPO were used for fermentation. After 36 h, TTMP output reached the highest value of 208.8 mg/L. The yield of TTMP using phosphoric acid-treated molasses as carbon source was 145.59% higher than control. Under the sulfuric acid treatment process of molasses (150 g), the maximum yield of TTMP was 895.13 mg/L, which was 183.18% higher than that of untreated molasses (316.1 mg/L). This study demonstrated that molasses is a high-quality and inexpensive carbon source for the manufacture of TTMP, laying the groundwork for the future industrial production of TTMP.

Citing Articles

Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis.

Liang S, Ling M, Li L, Gan S, Shang C Curr Microbiol. 2024; 82(1):33.

PMID: 39653804 DOI: 10.1007/s00284-024-03990-5.

Optimization of Molasses and Soybean Meal Content to Enhance Tetramethylpyrazine Yield by sp. TTMP20.

Li Y, Gan S, Luo L, Yang W, Mo L, Shang C Molecules. 2023; 28(18).

PMID: 37764292 PMC: 10535143. DOI: 10.3390/molecules28186515.

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