Synthesis of Carvacrol-Loaded Invasomes Nanoparticles Improved Acaricide Efficacy, Cuticle Invasion and Inhibition of Acetylcholinestrase Against Hard Ticks

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Mar 29

PMID 36985306

Authors

Amr Gamal

Shawky M Aboelhadid

Fatma I Abo El-Ela

Abdel-Azeem S Abdel-Baki

Samar M Ibrahium

Almahy M El-Mallah

Saleh Al-Quraishy

Ahmed O Hassan

Sahar M Gadelhaq

Affiliations

Soon will be listed here.

Abstract

Carvacrol is a monoterpenoid phenol found in many essential oils that has antibacterial, antifungal and antiparasitic activities. Drug loaded-invasome systems are used to deliver drugs utilizing nanoparticles to improve bioavailability, efficacy, and drug release duration. As a result, the present study developed carvacrol-loaded invasomes and evaluated their acaricidal effect against (cattle tick) and (dog tick). Carvacrol loaded-invasome (CLI) was prepared and characterized using UV/Vis spectrophotometer, zeta potential measurements, Scanning Transmission Electron Microscopy (STEM), Fourier Transform Infrared (FT-IR) Spectroscopy, and Differential Scanning Calorimetry Analysis. CLI (5%) induced significant mortality (100%) in adult ticks with LC of 2.60%, whereas the LC of pure carvacrol was 4.30%. Carvacrol and CLI were shown to have a significant larvicidal action on both tick species, with LCs of 0.24 and 0.21% against and 0.27 and 0.23% against , respectively. Carvacrol and CLI (5%) induced significant repellent activities for 24 h against and , as evidenced by the rod method and the petri-dish selective area choice method, respectively. High-performance liquid chromatography (HPLC) demonstrated that the CLI form had 3.86 times the permeability of pure carvacrol. Moreover, carvacrol and CLI inhibited acetylcholinesterase activity and decreased glutathione and malonedealdehyde levels in the treated ticks. In conclusion, invasomes significantly improved adulticidal and repellency activities of carvacrol against both tick species.

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