Isolation of Bioactive Metabolites from Soil Derived Fungus-

Fungi produce numerous secondary metabolites with intriguing biological properties for the health, industrial, and agricultural sectors. Herein, we report the high-yield isolation of phenolic natural products, N-formyl-4-hydroxyphenyl-acetamide (~117 mg/L) and atraric acid (~18 mg/L), from the ethyl acetate extract of the soil-derived fungus, . The structures of compounds and were elucidated through the detailed spectroscopic analysis of NMR and LCMS data. These compounds were assayed for their antimicrobial activities. It was observed that compounds and exhibited strong inhibition against a series of fungal strains but only weak antibacterial properties against multi-drug-resistant strains. More significantly, this is the first known instance of the isolation of atraric acid from a non-lichen fungal strain. We suggest the optimization of this fungal strain may exhibit elevated production of compounds and , potentially rendering it a valuable source for the industrial-scale production of these natural antimicrobial compounds. Further investigation is necessary to establish the veracity of this hypothesis.