Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury Via PI3K/Akt/mTOR Pathway

Overview

Journal Metabolites

Publisher MDPI

Date 2023 Mar 29

PMID 36984859

Authors

Ahmed M Fathi

Shaimaa Waz

Eman Alaaeldin

Nisreen D M Toni

Azza A K El-Sheikh

Ahmed M Sayed

Usama Ramadan Abdelmohsen

Maiiada H Nazmy

Affiliations

Soon will be listed here.

Abstract

Aims: To achieve site-specific delivery of blackberry leaves extract () loaded AgNPs to the hepatocyte in IRI and to verify possible molecular mechanisms.

Methods: IRI was induced in male Wister rats. Liver injury, hepatic histology, oxidative stress markers, hepatic expression of apoptosis-related proteins were evaluated. Non-targeted metabolomics for chemical characterization of blackberry leaves extract was performed.

Key Findings: Pre-treatment with protected against the deterioration caused by I/R, depicted by a significant improvement of liver functions and structure, as well as reduction of oxidative stress with a concomitant increase in antioxidants. Additionally, promoted phosphorylation of antiapoptotic proteins; PI3K, Akt and mTOR, while apoptotic proteins; Bax, Casp-9 and cleaved Casp-3 expressions were decreased. LC-HRMS-based metabolomics identified a range of metabolites, mainly flavonoids and anthocyanins. Upon comprehensive virtual screening and molecular dynamics simulation, the major annotated anthocyanins, cyanidin and pelargonidin glucosides, were suggested to act as PLA2 inhibitors.

Significance: can ameliorate hepatic IRI augmented by -AgNPs nano-formulation via suppressing, oxidative stress and apoptosis as well as stimulation of PI3K/Akt/mTOR signaling pathway.

Citing Articles

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