Mitochondrial Haemoglobin Is Upregulated with Hypoxia in Skeletal Muscle and Has a Conserved Interaction with ATP Synthase and Inhibitory Factor 1

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2023 Mar 29

PMID 36980252

Authors

Brad Ebanks

Gunjan Katyal

Chris Taylor

Adam Dowle

Chiara Papetti

Magnus Lucassen

Nicoleta Moisoi

Lisa Chakrabarti

Affiliations

Soon will be listed here.

Abstract

The globin protein superfamily has diverse functions. Haemoglobin has been found in non-erythroid locations, including within the mitochondria. Using co-immunoprecipitation and in silico methods, we investigated the interaction of mitochondrial haemoglobin with ATP synthase and its associated proteins, including inhibitory factor 1 (IF1). We measured the expression of mitochondrial haemoglobin in response to hypoxia. In vitro and in silico evidence of interactions between mitochondrial haemoglobin and ATP synthase were found, and we report upregulated mitochondrial haemoglobin expression in response to hypoxia within skeletal muscle tissue. Our observations indicate that mitochondrial pH and ATP synthase activity are implicated in the mitochondrial haemoglobin response to hypoxia.

Citing Articles

Spatial multi-omics in whole skeletal muscle reveals complex tissue architecture.

Martinez Mir C, Pisterzi P, de Poorter I, Rilou M, van Kranenburg M, Heijs B Commun Biol. 2024; 7(1):1272.

PMID: 39369093 PMC: 11455876. DOI: 10.1038/s42003-024-06949-1.

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