The Anti-Biofilm Potential of Linalool, a Major Compound from , Against and Its Toxicity Assessment in

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2023 Mar 29

PMID 36978412

Authors

Sarath Praseetha

Swapna Thacheril Sukumaran

Mathew Dan

Akshaya Rani Augustus

Shunmugiah Karutha Pandian

Shiburaj Sugathan

Affiliations

Soon will be listed here.

Abstract

The anti-biofilm and anti-virulence potential of the essential oil (E.O.) extracted from M. Dan & Sathish was determined against . A crystal violet assay was employed to quantify the biofilm. Linalool, a monoterpene alcohol from the E.O., showed concentration-dependent biofilm inhibition, with a maximum of 91% at a concentration of 0.004% (/). The AlamarBlue assay also confirmed Linalool's non-bactericidal anti-biofilm efficacy (0.004%). Linalool treatment impeded micro-colony formation, mature biofilm architecture, surface coverage, and biofilm thickness and impaired cell surface hydrophobicity and EPS production. Cysteine protease synthesis was quantified using the Azocasein assay, and Linalool treatment augmented its production. This suggests that Linalool destabilizes the biofilm matrix. It altered the expression of core regulons , , , and , and genes associated with virulence and biofilm formation, such as , , , and , as revealed by qPCR analysis. Cytotoxicity analysis using human kidney cells (HEK) and the histopathological analysis in proved Linalool to be a druggable molecule against the biofilms formed by This is the first report on Linalool's anti-biofilm and anti-virulence potential against .

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References

Bates C, Montanez G, Woods C, Vincent R, Eichenbaum Z . Identification and characterization of a Streptococcus pyogenes operon involved in binding of hemoproteins and acquisition of iron. Infect Immun. 2003; 71(3):1042-55. PMC: 148835. DOI: 10.1128/IAI.71.3.1042-1055.2003. View

Cassar S, Adatto I, Freeman J, Gamse J, Iturria I, Lawrence C . Use of Zebrafish in Drug Discovery Toxicology. Chem Res Toxicol. 2019; 33(1):95-118. PMC: 7162671. DOI: 10.1021/acs.chemrestox.9b00335. View

Beema Shafreen R, Selvaraj C, Singh S, Pandian S . In silico and in vitro studies of cinnamaldehyde and their derivatives against LuxS in Streptococcus pyogenes: effects on biofilm and virulence genes. J Mol Recognit. 2014; 27(2):106-16. DOI: 10.1002/jmr.2339. View

Viszwapriya D, Subramenium G, Prithika U, Balamurugan K, Pandian S . Betulin inhibits virulence and biofilm of Streptococcus pyogenes by suppressing ropB core regulon, sagA and dltA. Pathog Dis. 2016; 74(7). DOI: 10.1093/femspd/ftw088. View

Tan L, Eccersley L, Sriskandan S . Current views of haemolytic streptococcal pathogenesis. Curr Opin Infect Dis. 2014; 27(2):155-64. DOI: 10.1097/QCO.0000000000000047. View

Stewart P, Bjarnsholt T . Risk factors for chronic biofilm-related infection associated with implanted medical devices. Clin Microbiol Infect. 2020; 26(8):1034-1038. DOI: 10.1016/j.cmi.2020.02.027. View

Alagu Lakshmi S, Bhaskar J, Krishnan V, Sethupathy S, Pandipriya S, Aruni W . Inhibition of biofilm and biofilm-associated virulence factor production in methicillin-resistant Staphylococcus aureus by docosanol. J Biotechnol. 2020; 317:59-69. DOI: 10.1016/j.jbiotec.2020.04.014. View

Elyemni M, Louaste B, Nechad I, Elkamli T, Bouia A, Taleb M . Extraction of Essential Oils of L. by Two Different Methods: Hydrodistillation and Microwave Assisted Hydrodistillation. ScientificWorldJournal. 2019; 2019:3659432. PMC: 6463580. DOI: 10.1155/2019/3659432. View

Subramenium G, Viszwapriya D, Iyer P, Balamurugan K, Pandian S . covR Mediated Antibiofilm Activity of 3-Furancarboxaldehyde Increases the Virulence of Group A Streptococcus. PLoS One. 2015; 10(5):e0127210. PMC: 4433207. DOI: 10.1371/journal.pone.0127210. View

10.

Courtney H, Ofek I, Penfound T, Nizet V, Pence M, Kreikemeyer B . Relationship between expression of the family of M proteins and lipoteichoic acid to hydrophobicity and biofilm formation in Streptococcus pyogenes. PLoS One. 2009; 4(1):e4166. PMC: 2613554. DOI: 10.1371/journal.pone.0004166. View

11.

Wijesundara N, Rupasinghe H . Essential oils from Origanum vulgare and Salvia officinalis exhibit antibacterial and anti-biofilm activities against Streptococcus pyogenes. Microb Pathog. 2018; 117:118-127. DOI: 10.1016/j.micpath.2018.02.026. View

12.

Vijayakumar K, Manigandan V, Jeyapragash D, Bharathidasan V, Anandharaj B, Sathya M . Eucalyptol inhibits biofilm formation of and its mediated virulence factors. J Med Microbiol. 2020; 69(11):1308-1318. DOI: 10.1099/jmm.0.001253. View

13.

Hu H, Johani K, Gosbell I, Jacombs A, Almatroudi A, Whiteley G . Intensive care unit environmental surfaces are contaminated by multidrug-resistant bacteria in biofilms: combined results of conventional culture, pyrosequencing, scanning electron microscopy, and confocal laser microscopy. J Hosp Infect. 2015; 91(1):35-44. DOI: 10.1016/j.jhin.2015.05.016. View

14.

Honda-Ogawa M, Ogawa T, Terao Y, Sumitomo T, Nakata M, Ikebe K . Cysteine proteinase from Streptococcus pyogenes enables evasion of innate immunity via degradation of complement factors. J Biol Chem. 2013; 288(22):15854-64. PMC: 3668742. DOI: 10.1074/jbc.M113.469106. View

15.

Guo F, Liang Q, Zhang M, Chen W, Chen H, Yun Y . Antibacterial Activity and Mechanism of Linalool against . Molecules. 2021; 26(1). PMC: 7796449. DOI: 10.3390/molecules26010245. View

16.

Gao Z, Van Nostrand J, Zhou J, Zhong W, Chen K, Guo J . Anti-listeria Activities of Linalool and Its Mechanism Revealed by Comparative Transcriptome Analysis. Front Microbiol. 2020; 10:2947. PMC: 6938037. DOI: 10.3389/fmicb.2019.02947. View

17.

Hill A, Teraoka H, Heideman W, Peterson R . Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol Sci. 2005; 86(1):6-19. DOI: 10.1093/toxsci/kfi110. View

18.

Elshikh M, Ahmed S, Funston S, Dunlop P, McGaw M, Marchant R . Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnol Lett. 2016; 38(6):1015-9. PMC: 4853446. DOI: 10.1007/s10529-016-2079-2. View

19.

Wojcik M, Eleftheriadis N, Zwinderman M, Domling A, Dekker F, Boersma Y . Identification of potential antivirulence agents by substitution-oriented screening for inhibitors of Streptococcus pyogenes sortase A. Eur J Med Chem. 2018; 161:93-100. DOI: 10.1016/j.ejmech.2018.10.027. View

20.

Graham M, Virtaneva K, Porcella S, Barry W, Gowen B, Johnson C . Group A Streptococcus transcriptome dynamics during growth in human blood reveals bacterial adaptive and survival strategies. Am J Pathol. 2005; 166(2):455-65. PMC: 1602339. DOI: 10.1016/S0002-9440(10)62268-7. View