4D Force Detection of Cell Adhesion and Contractility

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2023 Mar 28

PMID 36975035

Authors

Nafsika Chala

Xinyu Zhang

Tomaso Zambelli

Ziyi Zhang

Teseo Schneider

Daniele Panozzo

Dimos Poulikakos

Aldo Ferrari

Affiliations

Soon will be listed here.

Abstract

Mechanical signals establish two-way communication between mammalian cells and their environment. Cells contacting a surface exert forces via contractility and transmit them at the areas of focal adhesions. External stimuli, such as compressive and pulling forces, typically affect the adhesion-free cell surface. Here, we demonstrate the collaborative employment of Fluidic Force Microscopy and confocal Traction Force Microscopy supported by the Cellogram solver to enable a powerful integrated force probing approach, where controlled vertical forces are applied to the free surface of individual cells, while the concomitant deformations are used to map their transmission to the substrate. Force transmission across human cells is measured with unprecedented temporal and spatial resolution, enabling the investigation of the cellular mechanisms involved in the adaptation, or maladaptation, to external mechanical stimuli. Altogether, the system enables facile and precise force interrogation of individual cells, with the capacity to perform population-based analysis.

Citing Articles

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