Comparative Evaluation of LAMP and Nested PCR for the Rapid Diagnosis of Infection

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 Mar 27

PMID 36969943

Authors

Yumiao Feng

Miaomiao Wang

Haiqin Jiang

Ying Shi

Wenyue Zhang

Hongsheng Wang

Affiliations

Soon will be listed here.

Abstract

Purpose: Culture of is very time-consuming, taking several weeks to produce positive results. Seeking rapid and sensitive diagnostic methods for diagnosis can greatly improve patient treatment. Our study aimed to compare the rapid diagnostic abilities of polymerase chain reaction (PCR), nested PCR and loop mediated isothermal amplification (LAMP) of detecting in skin samples from patients with infection.

Methods: A total of 6 strains and 6 skin samples with definite diagnosis of infection were included in the study. We optimized LAMP performance for detection of genomic DNA and confirmed the specificity of the primers. Then, the sensitivity of the LAMP and nested PCR assays were assessed by strains and clinical samples.

Results: Nested PCR was 10-fold more sensitive than the LAMP assay by serial dilution of DNA. PCR positive samples were all positive by LAMP detection of 6 clinical strains. Out of 6 clinical skin specimens confirmed as infection, 0 (0%), 3 (50%), 3 (50%), and 4 (66.6%) were positive by PCR, nested PCR, LAMP and culture. The LAMP shared the same sensitivity than nested PCR in strains and clinical samples, but it was easy to perform and faster than nested PCR assay.

Conclusion: Compared with conventional PCR, LAMP and nested PCR are more sensitive and have a higher detection rate of in clinical skin specimens. The LAMP assay proved to be more suitable for rapid diagnosis of infection in a shorter time, especially in resource-limited settings.

Citing Articles

A Rapid PCR-Based Diagnostic Method for Skin Infection with .

Li Y, Feng Y, Li D, Shi D, Chen G Infect Drug Resist. 2024; 17:2833-2851.

PMID: 39005849 PMC: 11246082. DOI: 10.2147/IDR.S463798.

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