Simultaneous Quantitative Analysis and Anti-arthritic Effects of Five Polyphenols from

Overview

Journal Front Physiol

Date 2023 Mar 27

PMID 36969583

Authors

Fang Liu

Shipeng Zhan

Pu Zhang

Changsheng Jia

Qingzong Zhu

Qing Dai

Mingjie Yu

Lin Cheng

Lirong Xiong

Fengjun Sun

Peiyuan Xia

Xiao Zhang

Jing Hu

Affiliations

Soon will be listed here.

Abstract

The fruit of has been widely used for a thousand years for treating diarrhea, ulcers, and arthritic diseases in Asian countries. However, the active components of this Traditional Chinese medicine and their mechanisms remain unclear, necessitating further investigation. To perform simultaneous quantitative analysis of five polyphenols in and evaluate their anti-arthritic effects including antioxidant and anti-inflammatory activity . Water, 50% water-ethanol, and pure ethanol were used as extract solvents. Quantitative analysis of gallic acid, corilagin, chebulanin, chebulagic acid, and ellagic acid in the three extracts was performed using high-performance liquid chromatography (HPLC). Antioxidant activity was assessed by the 2,2-diphenylpicrylhydrazyl (DPPH) radical-scavenging assay, and anti-inflammatory activity was evaluated by detecting interleukin (IL)-6 and IL-8 expression in IL-1β-stimulated MH7A cells. The 50% water-ethanol solvent was the optimal solvent yielding the highest total polyphenol content, and the concentrations of chebulanin and chebulagic acid were much higher than those of gallic acid, corilagin, and ellagic acid in the extracts. The DPPH radical-scavenging assay showed that gallic acid and ellagic acid were the strongest antioxidative components, while the other three components showed comparable antioxidative activity. As for the anti-inflammatory effect, chebulanin and chebulagic acid significantly inhibited IL-6 and IL-8 expression at all three concentrations; corilagin and ellagic acid significantly inhibited IL-6 and IL-8 expression at high concentration; and gallic acid could not inhibit IL-8 expression and showed weak inhibition of IL-6 expression in IL-1β-stimulated MH7A cells. Principal component analysis indicated that chebulanin and chebulagic acid were the main components responsible for the anti-arthritic effects of . Our findings highlight the potential anti-arthritic role of chebulanin and chebulagic acid from .

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References

Zhou X, Han L, Ye Y, Wang R, Zhang Y, Bai C . [Progress on treatment of rheumatoid arthritis with ethnodrugs]. Zhongguo Zhong Yao Za Zhi. 2017; 42(12):2398-2407. DOI: 10.19540/j.cnki.cjcmm.2017.0115. View

Eid O, Elkady W, Ezzat S, El Sayed A, Abd Elsattar E . Comprehensive Overview: The Effect of Using Different Solvents for Barley Extraction with Its Anti-Inflammatory and Antioxidant Activity. Chem Biodivers. 2023; 20(3):e202200935. DOI: 10.1002/cbdv.202200935. View

Weyand C, Goronzy J . The immunology of rheumatoid arthritis. Nat Immunol. 2020; 22(1):10-18. PMC: 8557973. DOI: 10.1038/s41590-020-00816-x. View

Georganas C, Liu H, Perlman H, Hoffmann A, Thimmapaya B, Pope R . Regulation of IL-6 and IL-8 expression in rheumatoid arthritis synovial fibroblasts: the dominant role for NF-kappa B but not C/EBP beta or c-Jun. J Immunol. 2000; 165(12):7199-206. DOI: 10.4049/jimmunol.165.12.7199. View

Nigam M, Mishra A, Adhikari-Devkota A, Dirar A, Hassan M, Adhikari A . Fruits of Terminalia chebula Retz.: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Phytother Res. 2020; 34(10):2518-2533. DOI: 10.1002/ptr.6702. View

Ainsworth E, Gillespie K . Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007; 2(4):875-7. DOI: 10.1038/nprot.2007.102. View

Karlapudi V, Prasad Mungara A, Sengupta K, Davis B, Raychaudhuri S . A Placebo-Controlled Double-Blind Study Demonstrates the Clinical Efficacy of a Novel Herbal Formulation for Relieving Joint Discomfort in Human Subjects with Osteoarthritis of Knee. J Med Food. 2018; 21(5):511-520. DOI: 10.1089/jmf.2017.0065. View

Li J, Li J, Yue Y, Hu Y, Cheng W, Liu R . Genistein suppresses tumor necrosis factor α-induced inflammation via modulating reactive oxygen species/Akt/nuclear factor κB and adenosine monophosphate-activated protein kinase signal pathways in human synoviocyte MH7A cells. Drug Des Devel Ther. 2014; 8:315-23. PMC: 3962316. DOI: 10.2147/DDDT.S52354. View

Pfundstein B, El Desouky S, Hull W, Haubner R, Erben G, Owen R . Polyphenolic compounds in the fruits of Egyptian medicinal plants (Terminalia bellerica, Terminalia chebula and Terminalia horrida): characterization, quantitation and determination of antioxidant capacities. Phytochemistry. 2010; 71(10):1132-48. DOI: 10.1016/j.phytochem.2010.03.018. View

10.

Manosroi A, Jantrawut P, Ogihara E, Yamamoto A, Fukatsu M, Yasukawa K . Biological activities of phenolic compounds and triterpenoids from the galls of Terminalia chebula. Chem Biodivers. 2013; 10(8):1448-63. DOI: 10.1002/cbdv.201300149. View

11.

Chen Z, Bozec A, Ramming A, Schett G . Anti-inflammatory and immune-regulatory cytokines in rheumatoid arthritis. Nat Rev Rheumatol. 2018; 15(1):9-17. DOI: 10.1038/s41584-018-0109-2. View

12.

McInnes I, Buckley C, Isaacs J . Cytokines in rheumatoid arthritis - shaping the immunological landscape. Nat Rev Rheumatol. 2015; 12(1):63-8. DOI: 10.1038/nrrheum.2015.171. View

13.

Nair V, Singh S, Gupta Y . Anti-arthritic and disease modifying activity of Terminalia chebula Retz. in experimental models. J Pharm Pharmacol. 2010; 62(12):1801-6. DOI: 10.1111/j.2042-7158.2010.01193.x. View

14.

Miyazawa K, Mori A, Okudaira H . Establishment and characterization of a novel human rheumatoid fibroblast-like synoviocyte line, MH7A, immortalized with SV40 T antigen. J Biochem. 1998; 124(6):1153-62. DOI: 10.1093/oxfordjournals.jbchem.a022233. View

15.

Jang S, Kwon E, Lee J . Rheumatoid Arthritis: Pathogenic Roles of Diverse Immune Cells. Int J Mol Sci. 2022; 23(2). PMC: 8780115. DOI: 10.3390/ijms23020905. View

16.

Kumar R, Kumar R, Khursheed R, Awasthi A, Ramanunny A, Kaur J . Validated Reverse Phase-High-Performance Liquid Chromatography Method for Estimation of Fisetin in Self-Nanoemulsifying Drug Delivery System. Assay Drug Dev Technol. 2020; 18(6):274-281. DOI: 10.1089/adt.2020.983. View

17.

Liu F, Liu Y, Zhan S, Lv J, Sun F, Weng B . Chebulanin exerts its anti-inflammatory and anti-arthritic effects via inhibiting NF-κB and MAPK activation in collagen-induced arthritis mice. Int Immunopharmacol. 2020; 88:106823. DOI: 10.1016/j.intimp.2020.106823. View

18.

Zhang Q, Peng W, Wei S, Wei D, Li R, Liu J . Guizhi-Shaoyao-Zhimu decoction possesses anti-arthritic effects on type II collagen-induced arthritis in rats via suppression of inflammatory reactions, inhibition of invasion & migration and induction of apoptosis in synovial fibroblasts. Biomed Pharmacother. 2019; 118:109367. DOI: 10.1016/j.biopha.2019.109367. View

19.

Gulcin I . Antioxidants and antioxidant methods: an updated overview. Arch Toxicol. 2020; 94(3):651-715. DOI: 10.1007/s00204-020-02689-3. View

20.

Yang M, Ali Z, Khan I, Khan S . Anti-inflammatory activity of constituents isolated from Terminalia chebula. Nat Prod Commun. 2014; 9(7):965-8. View