MicroRNAs Emerging Coordinate with Placental Mammals Alter Pathways in Endometrial Epithelia Important for Endometrial Function

Overview

Journal iScience

Publisher Cell Press

Date 2023 Mar 27

PMID 36968081

Authors

Laura Hume

Jessica C Edge

Haidee Tinning

Dapeng Wang

Alysha S Taylor

Vladimir Ovchinnikov

Annika V Geijer-Simpson

Pavle Vrljicak

Jan J Brosens

Emma S Lucas

Nigel A B Simpson

Jayne Shillito

Karen Forbes

Mary J OConnell

Niamh Forde

Affiliations

Soon will be listed here.

Abstract

We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 μg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss.

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