Impact of Spatial Metabolomics on Immune-microenvironment in Oral Cancer Prognosis: a Clinical Report

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2023 Mar 26

PMID 36966422

Authors

Swarnendu Bag

Janina Oetjen

Soni Shaikh

Amrita Chaudhary

Pattatheyil Arun

Geetashree Mukherjee

Affiliations

Soon will be listed here.

Abstract

MALDI imaging for metabolites and immunohistochemistry for 38 immune markers was used to characterize the spatial biology of 2 primary oral tumours, one from a patient with an early recurrence (Tumour R), and the other from a patient with no recurrence 2 years after treatment completion (Tumour NR). Tumour R had an increased purine nucleotide metabolism in different regions of tumour and adenosine-mediated suppression of immune cells compared to Tumour NR. The differentially expressed markers in the different spatial locations in tumour R were CD33, CD163, TGF-β, COX2, PD-L1, CD8 and CD20. These results suggest that altered tumour metabolomics concomitant with a modified immune microenvironment could be a potential marker of recurrence.

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References

Hu Y, Zhong R, Li H, Zou Y . Effects of Betel Quid, Smoking and Alcohol on Oral Cancer Risk: A Case-Control Study in Hunan Province, China. Subst Use Misuse. 2020; 55(9):1501-1508. DOI: 10.1080/10826084.2020.1750031. View

Hanahan D . Hallmarks of Cancer: New Dimensions. Cancer Discov. 2022; 12(1):31-46. DOI: 10.1158/2159-8290.CD-21-1059. View

Martinez-Reyes I, Chandel N . Cancer metabolism: looking forward. Nat Rev Cancer. 2021; 21(10):669-680. DOI: 10.1038/s41568-021-00378-6. View

Hinshaw D, Shevde L . The Tumor Microenvironment Innately Modulates Cancer Progression. Cancer Res. 2019; 79(18):4557-4566. PMC: 6744958. DOI: 10.1158/0008-5472.CAN-18-3962. View

Annaratone L, Cascardi E, Vissio E, Sarotto I, Chmielik E, Sapino A . The Multifaceted Nature of Tumor Microenvironment in Breast Carcinomas. Pathobiology. 2020; 87(2):125-142. PMC: 7265767. DOI: 10.1159/000507055. View

Sistigu A, Musella M, Galassi C, Vitale I, De Maria R . Tuning Cancer Fate: Tumor Microenvironment's Role in Cancer Stem Cell Quiescence and Reawakening. Front Immunol. 2020; 11:2166. PMC: 7609361. DOI: 10.3389/fimmu.2020.02166. View

Leone R, Powell J . Metabolism of immune cells in cancer. Nat Rev Cancer. 2020; 20(9):516-531. PMC: 8041116. DOI: 10.1038/s41568-020-0273-y. View

Bayik D, Lathia J . Cancer stem cell-immune cell crosstalk in tumour progression. Nat Rev Cancer. 2021; 21(8):526-536. PMC: 8740903. DOI: 10.1038/s41568-021-00366-w. View

Wang Z, Peng W, Zhang P, Yang X, Zhou Q . Lactate in the tumour microenvironment: From immune modulation to therapy. EBioMedicine. 2021; 73:103627. PMC: 8524104. DOI: 10.1016/j.ebiom.2021.103627. View

10.

Puccetti P, Fallarino F . T cell fat catabolism: A novel target for kynurenine?. EBioMedicine. 2021; 75:103779. PMC: 8718980. DOI: 10.1016/j.ebiom.2021.103779. View

11.

Mukherjee G, Bag S, Chakraborty P, Dey D, Roy S, Jain P . Density of CD3+ and CD8+ cells in gingivo-buccal oral squamous cell carcinoma is associated with lymph node metastases and survival. PLoS One. 2020; 15(11):e0242058. PMC: 7676650. DOI: 10.1371/journal.pone.0242058. View

12.

Hendry S, Salgado R, Gevaert T, Russell P, John T, Thapa B . Assessing Tumor-infiltrating Lymphocytes in Solid Tumors: A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in.... Adv Anat Pathol. 2017; 24(5):235-251. PMC: 5564448. DOI: 10.1097/PAP.0000000000000162. View

13.

Hendry S, Salgado R, Gevaert T, Russell P, John T, Thapa B . Assessing Tumor-Infiltrating Lymphocytes in Solid Tumors: A Practical Review for Pathologists and Proposal for a Standardized Method from the International Immuno-Oncology Biomarkers Working Group: Part 2: TILs in Melanoma, Gastrointestinal Tract.... Adv Anat Pathol. 2017; 24(6):311-335. PMC: 5638696. DOI: 10.1097/PAP.0000000000000161. View

14.

Deininger S, Cornett D, Paape R, Becker M, Pineau C, Rauser S . Normalization in MALDI-TOF imaging datasets of proteins: practical considerations. Anal Bioanal Chem. 2011; 401(1):167-81. PMC: 3124646. DOI: 10.1007/s00216-011-4929-z. View

15.

Leone R, Emens L . Targeting adenosine for cancer immunotherapy. J Immunother Cancer. 2018; 6(1):57. PMC: 6006764. DOI: 10.1186/s40425-018-0360-8. View

16.

Semenza G . Intratumoral Hypoxia and Mechanisms of Immune Evasion Mediated by Hypoxia-Inducible Factors. Physiology (Bethesda). 2021; 36(2):73-83. DOI: 10.1152/physiol.00034.2020. View

17.

Chaudhary A, Bag S, Arora N, Radhakrishnan V, Mishra D, Mukherjee G . Hypoxic Transformation of Immune Cell Metabolism Within the Microenvironment of Oral Cancers. Front Oral Health. 2022; 1:585710. PMC: 8757756. DOI: 10.3389/froh.2020.585710. View

18.

Yadollahi P, Jeon Y, Ng W, Choi I . Current understanding of cancer-intrinsic PD-L1: regulation of expression and its protumoral activity. BMB Rep. 2020; 54(1):12-20. PMC: 7851443. View

19.

DiLillo D, Yanaba K, Tedder T . B cells are required for optimal CD4+ and CD8+ T cell tumor immunity: therapeutic B cell depletion enhances B16 melanoma growth in mice. J Immunol. 2010; 184(7):4006-16. PMC: 3733120. DOI: 10.4049/jimmunol.0903009. View

20.

Nielsen J, Sahota R, Milne K, Kost S, Nesslinger N, Watson P . CD20+ tumor-infiltrating lymphocytes have an atypical CD27- memory phenotype and together with CD8+ T cells promote favorable prognosis in ovarian cancer. Clin Cancer Res. 2012; 18(12):3281-92. DOI: 10.1158/1078-0432.CCR-12-0234. View