Label-free Photoelectrochemical Immunosensing of α-fetoprotein Based on Eu-TiO Nanocomposites Sensitized with Dye-encapsulated HMA

Overview

Journal Anal Sci

Publisher Springer

Specialty Chemistry

Date 2023 Mar 24

PMID 36961621

Authors

Kun Wang

Xue Gao

Jianfeng Chen

Xiaoyan Yang

Affiliations

Soon will be listed here.

Abstract

In this study, a sensitive photoelectrochemical immunosensor with dye-enhanced anodic photocurrent response was proposed for sensitive detection of α-fetoprotein (AFP). Specifically, europium-doped TiO (Eu-TiO) was used as the photoelectrochemical functional material and coated onto indium tin oxide (ITO) electrode. Doxorubicin (DOX) as an excellent fluorescent dye was encapsulated in the hydrophobically modified alginate (HMA). Then the dye-loaded HMA was modified onto the surface of Eu-TiO to further sensitize the photocurrent response. The results showed that the photoelectrical signal was enhanced and stabilized due to the effect of sensitization of DOX on Eu-TiO material. The constructed PEC sensor revealed a good linear response to AFP antigen ranging from 0.5 to 100 ng/mL with a detection limit of 0.41 pg/mL. The clinical patient's serum test results obtained from the proposed PEC immunosensor were consistent with those obtained from the commercial electrochemilunescence assay. The proposed PEC sensing method could be a promising analytical tool for the detection of AFP in clinical analysis.

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